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In this study, hydrogels are prepared by decellularization of human placental tissue using detergent and enzymes and by the subsequent creation of a homogenized acellular placental tissue powder (P\u2010ECM). A perfusion\u2010based decellularization approach is employed using detergent and enzymes. The P\u2010ECM with and without gamma irradiation is then utilized to prepare P\u2010ECM hydrogels. Physical and biological evaluations are conducted to assess the suitability of the P\u2010ECM hydrogels for biocompatibility. The decellularized tissue has significantly reduced cellular content and retains the major ECM proteins. Increasing the concentration of P\u2010ECM leads to improved mechanical properties of the P\u2010ECM hydrogels. The biocompatibility of the P\u2010ECM hydrogel is demonstrated through cell proliferation and viability assays. Notably, gamma\u2010sterilized P\u2010ECM does not support the formation of a stable hydrogel. Nonetheless, the use of HCl during the digestion process effectively decreases spore growth and bacterial bioburden. The study demonstrates that P\u2010ECM hydrogels exhibit physical and biological attributes conducive to soft tissue reconstruction. These hydrogels establish a favorable microenvironment for cell growth and the need for investigating innovative sterilization methods.<\/jats:p>","DOI":"10.1002\/adbi.202300349","type":"journal-article","created":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T01:24:45Z","timestamp":1696296285000},"source":"Crossref","is-referenced-by-count":15,"title":["Biocompatible Human Placental Extracellular Matrix Derived Hydrogels"],"prefix":"10.1002","volume":"8","author":[{"given":"Sunil","family":"Gujjar","sequence":"first","affiliation":[{"name":"Biomaterials Laboratory Translational Health Science and Technology Institute  Faridabad Haryana 121001 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anurag","family":"Tyagi","sequence":"additional","affiliation":[{"name":"Biomaterials Laboratory Translational Health Science and Technology Institute  Faridabad Haryana 121001 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