{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T18:15:46Z","timestamp":1781028946285,"version":"3.54.1"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,12,24]],"date-time":"2023-12-24T00:00:00Z","timestamp":1703376000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81703975"],"award-info":[{"award-number":["81703975"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Advanced Biology"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cerebral infarction is one of the most common diseases for aged people. Compound Tongluo Decoction (CTLD), a classic traditional Chinese Medicine prescription, has been widely used in the treatment of ischemic cerebral infarction. Transient middle cerebral artery occlusion (tMCAO) rat model is established for the animal experiment and oxygen\u2010glucose deprivation and reperfusion (OGD\/R) human umbilical vein endothelial cells (HUVECs) model are established for the cell experiment. This also use Nrf2<jats:sup>\u2010\/\u2010<\/jats:sup> rats to detect the role of nuclear factor erythroid 2\u2010related factor 2 (Nrf2). Longa score, Evans blue staining, brain water content measurement, and histological observation are done. The levels of reactive oxygen species (ROS), malondialdehyde (MDA), superoxide dismutase (SOD), and other ferroptosis\u2010related components are detected respectively. In the vivo experiment, CTLD relieved ischemia\u2010reperfusion (IR) injury symptoms and attenuated IR injury in brain tissues of tMCAO rats by relieving peroxidation injury in brain tissues and inhibiting ferroptosis in tMCAO rats. Moreover, CTLD reversed OGD\/R\u2010induced oxidative damage of endothelial cells via suppressing ferroptosis. After knocking out the Nrf2 gene, the protective effect of CTLD is sharply reduced. This study put forward that CTLD can inhibit ferroptosis in I\/R\u2010injured vascular endothelium by regulating Nrf2\/ARE\/SLC7A11 signaling to improve the relative symptoms of rats after cerebral I\/R injury, thus providing a viable treatment option for cerebrovascular disease.<\/jats:p>","DOI":"10.1002\/adbi.202300416","type":"journal-article","created":{"date-parts":[[2023,12,24]],"date-time":"2023-12-24T23:20:33Z","timestamp":1703460033000},"source":"Crossref","is-referenced-by-count":9,"title":["Protective Effect of Compound Tongluo Decoction on Brain Vascular Endothelial Cells after Ischemia\u2010Reperfusion by Inhibition of Ferroptosis Through Regulating Nrf2\/ARE\/SLC7A11 Signaling Pathway"],"prefix":"10.1002","volume":"8","author":[{"given":"Peiyi","family":"Li","sequence":"first","affiliation":[{"name":"School of Chinese Medicine School of Integrated Chinese and Western Medicine Nanjing University of Chinese Medicine  Nanjing 210023 P. R. China"},{"name":"The First School of Clinical Medicine Nanjing University of Chinese Medicine  Nanjing 210023 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongda","family":"Wang","sequence":"additional","affiliation":[{"name":"Affiliated Hospital of Integrated Traditional Chinese and Western Medicine Nanjing University of Chinese Medicine  Nanjing 210028 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Chinese Medicine School of Integrated Chinese and Western Medicine Nanjing University of Chinese Medicine  Nanjing 210023 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolan","family":"Cheng","sequence":"additional","affiliation":[{"name":"School of Chinese Medicine School of Integrated Chinese and Western Medicine Nanjing University of Chinese Medicine  Nanjing 210023 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhennian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Neurology Nanjing Hospital of Chinese Medicine affiliated to Nanjing University of Chinese Medicine  Nanjing Jiangsu 210001 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingqing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurology Nanjing Hospital of Chinese Medicine affiliated to Nanjing University of Chinese Medicine  Nanjing Jiangsu 210001 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sulei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurology Nanjing Hospital of Chinese Medicine affiliated to Nanjing University of Chinese Medicine  Nanjing Jiangsu 210001 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiou","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Children's Hospital of Nanjing Medical University  Nanjing Jiangsu 210017 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1761-1517","authenticated-orcid":false,"given":"Zhen","family":"Hui","sequence":"additional","affiliation":[{"name":"Department of Neurology Nanjing Hospital of Chinese Medicine affiliated to Nanjing University of Chinese Medicine  Nanjing Jiangsu 210001 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,12,24]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1159\/000489241"},{"key":"e_1_2_10_2_1","volume-title":"Ch18 Mechanisms of Vascular Disease","author":"Cowled P.","year":"2011"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/9929687"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gmv086"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2019.101107"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2015.158"},{"key":"e_1_2_10_7_1","volume":"115","author":"Liu P.","year":"2018","journal-title":"Proc Natl Acad Sci"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-021-03452-x"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-021-03042-7"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2020.101503"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05293"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2017.465"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2016.06.006"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000337155"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2018.00466"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2020.112616"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00452.2017"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.03.042"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2015.06.011"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.29219"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2015.11.003"},{"key":"e_1_2_10_22_1","first-page":"5405","volume":"15","author":"Hao S.","year":"2018","journal-title":"Oncol Lett"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10637-021-01070-0"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-03362-4"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2020.105450"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaw9872"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-021-04307-1"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2019.05.006"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.109048"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.38554"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.47651"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12700"}],"container-title":["Advanced Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adbi.202300416","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T15:49:30Z","timestamp":1759420170000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adbi.202300416"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,24]]},"references-count":32,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,3]]}},"alternative-id":["10.1002\/adbi.202300416"],"URL":"https:\/\/doi.org\/10.1002\/adbi.202300416","archive":["Portico"],"relation":{},"ISSN":["2701-0198","2701-0198"],"issn-type":[{"value":"2701-0198","type":"print"},{"value":"2701-0198","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,24]]},"article-number":"2300416"}}