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While many methods to detect this loss have been developed, enhancing the field, these existing methods often suffer from being labor\u2010intensive, expensive, and not sufficiently sensitive. To bridge this gap, a novel and more efficient technique is developed, named the SinChro assay. This method employs multiplexed single\u2010cell droplet PCR, designed to detect cells with sex chromosome loss at single\u2010cell resolution. Through the SinChro assay, the age\u2010dependent increase in Y chromosome loss in male blood is successfully mapped. The age\u2010dependent loss of the X chromosome in female blood is also identified, a finding that has been challenging with existing methods. The advent of the SinChro assay marks a significant breakthrough in the study of age\u2010related sex mosaicism. Its utility extends beyond blood analysis, applicable to a variety of tissues, and it holds the potential to deepen the understanding of biological aging and related diseases.<\/jats:p>","DOI":"10.1002\/adbi.202300512","type":"journal-article","created":{"date-parts":[[2024,4,29]],"date-time":"2024-04-29T22:24:32Z","timestamp":1714429472000},"source":"Crossref","is-referenced-by-count":5,"title":["A Novel Method for Acquired Sex Chromosome Mosaicism"],"prefix":"10.1002","volume":"8","author":[{"given":"Junichi","family":"Tsuchiya","sequence":"first","affiliation":[{"name":"TL Genomics Inc.  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