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The purpose of this investigation is to evaluate the effect of different intensities of in vivo cold\u2010water immersion and in vitro cold exposure on autophagic and apoptotic signaling in young and older males. Peripheral blood mononuclear cells (PBMCs) are isolated at baseline, end\u2010cold exposure, and after 3\u00a0h of thermoneutral recovery. Additionally, PBMCs are treated with rapamycin and bafilomycin prior to in vitro cold exposure equivalent to in vivo core temperatures (35\u201337\u00a0\u00b0C). Proteins associated with autophagy, apoptosis, the heat shock response, and inflammation are analyzed via Western blotting. Moderate cold stress (0.5\u00a0\u00b0C decrease in core temperature) increased autophagic and heat shock protein activity while high cold stress (1.0\u00a0\u00b0C decrease in core temperature) augmented apoptosis in young males. In older males, minimal autophagic activation during both cold\u2010water exposures are associated with increased apoptotic and inflammatory proteins. Although in vitro cold exposure confirmed age\u2010related dysfunction in autophagy, rapamycin\u2010induced stimulation of autophagic proteins underlie the potential to reverse age\u2010related vulnerability to cold exposure.<\/jats:p>","DOI":"10.1002\/adbi.202300560","type":"journal-article","created":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T16:16:11Z","timestamp":1703693771000},"source":"Crossref","is-referenced-by-count":12,"title":["Temperature\u2010Dependent Relationship of Autophagy and Apoptotic Signaling During Cold\u2010Water Immersion in Young and Older Males"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6232-2860","authenticated-orcid":false,"given":"Kelli E.","family":"King","sequence":"first","affiliation":[{"name":"Human and Environmental Physiology Research Unit School of Human Kinetics University of Ottawa  125 University Pvt, Monpetit Hall, Room 367 Ottawa K1N 6N5 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2729-2077","authenticated-orcid":false,"given":"James J.","family":"McCormick","sequence":"additional","affiliation":[{"name":"Human and Environmental Physiology Research Unit School of Human Kinetics University of Ottawa  125 University Pvt, Monpetit Hall, Room 367 Ottawa K1N 6N5 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8683-6973","authenticated-orcid":false,"given":"Glen P.","family":"Kenny","sequence":"additional","affiliation":[{"name":"Human and Environmental Physiology Research Unit School of Human Kinetics University of Ottawa  125 University Pvt, Monpetit Hall, Room 367 Ottawa K1N 6N5 Canada"},{"name":"Clinical Epidemiology Program Ottawa Hospital Research Institute  1053 Carling Ave Ottawa ON K1Y 4E9 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,12,27]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2002-20137"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01088.2005"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1113\/EP087718"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph19010501"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2009.07.005"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2020.1797280"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a004440"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.202932"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2014.150"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2152"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2003.08.005"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.462408"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0BM00488J"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.05.037"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-018-0235-8"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.30856"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP277091"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910570321"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.576463"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2022.822348"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-27537-1_49"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0128976"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtherbio.2020.102643"},{"key":"e_1_2_9_24_1","author":"McCormick J. 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