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TLR\u20102 activation contributes to the degenerative process by increasing the expression of extracellular matrix\u2010degrading enzymes, pro\u2010inflammatory cytokines, and neurotrophins. As potent post\u2010transcriptional regulators, microRNAs can modulate intracellular mechanisms, and their dysregulation is known to contribute to numerous pathologies. This study aims to investigate the impact of TLR\u20102 signaling on miRNA dysregulation in the context of IVD degeneration. Small\u2010RNA sequencing of degenerated IVD cells shows the dysregulation of ten miRNAs following TLR\u20102 activation by PAM2CSK4. The miR\u2010155\u20105p is most significantly upregulated in degenerated and non\u2010degenerated annulus fibrosus and nucleus pulposus cells. Sequence\u2010based target and pathway prediction shows the involvement of miR\u2010155\u20105p in inflammation\u2010 and cell fate\u2010related pathways and TLR\u20102\u2010induced miR\u2010155\u20105p expression leads to the downregulation of its target c\u2010FOS. Furthermore, changes specific to the activation of TLR\u20102 through fragmented fibronectin are seen in miR\u2010484 and miR\u2010487. Lastly, miR\u2010100\u20103p, miR\u2010320b, and miR\u2010181a\u20103p expression exhibit degeneration\u2010dependent changes. These results show that TLR\u20102 signaling leads to the dysregulation of miRNAs in IVD cells as well as their possible downstream effects on inflammation and degeneration. The identified miRNAs provide important opportunities as potential therapeutic targets for IVD degeneration and low back pain.<\/jats:p>","DOI":"10.1002\/adbi.202300581","type":"journal-article","created":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T02:05:19Z","timestamp":1709172319000},"source":"Crossref","is-referenced-by-count":4,"title":["Exploring the Impact of TLR\u20102 Signaling on miRNA Dysregulation in Intervertebral Disc Degeneration"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-3114-951X","authenticated-orcid":false,"given":"Petra","family":"Cazzanelli","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering Rochester Institute of Technology  160 Lomb Memorial Drive Rochester NY 14623 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikkael","family":"Lamoca","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Rochester Institute of Technology  160 Lomb 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