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Young (17\u201326 weeks) and aged (64\u201372 weeks) male C57BL\/6J mice (<jats:italic>n<\/jats:italic>\u00a0=\u00a072 in total, <jats:italic>n<\/jats:italic>\u00a0=\u00a06 per group) are allocated into 3 groups: the fracture group (Fx) undergoes femur osteotomy stabilized via external fixator, the combined trauma group (THFx) additionally receives pressure\u2010controlled trauma hemorrhage (TH) and Sham animals are implanted with catheter and fixator without blood loss or osteotomy. Femoral bones are evaluated histologically 24\u00a0h and 3 weeks post\u2010trauma, while RANKL\/OPG and \u03b2\u2010CTx are measured systemically via ELISA after 3 weeks. Aging results in less mineralized bone and fewer osteoclasts within the fracture of aged mice in contrast to young groups after three weeks. Systemically, aged animals exhibit increased RANKL and OPG levels after fracture compared to their young counterparts. The RANKL\/OPG ratio rises in aged Fx animals compared to young mice, with a similar trend in THFx groups. In conclusion, age has an effect during the later course of fracture healing on the cellular and systemic levels.<\/jats:p>","DOI":"10.1002\/adbi.202300653","type":"journal-article","created":{"date-parts":[[2024,8,21]],"date-time":"2024-08-21T19:55:41Z","timestamp":1724270141000},"source":"Crossref","is-referenced-by-count":7,"title":["Aging Influences Fracture Healing on the Cellular Level and Alters Systemic RANKL and OPG Concentrations in a Murine Model"],"prefix":"10.1002","volume":"8","author":[{"given":"Tianqi","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Trauma Surgery Hannover Medical School  Carl\u2010Neuberg\u2010Str. 1 30625 Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Neunaber","sequence":"additional","affiliation":[{"name":"Department of Trauma Surgery Hannover Medical School  Carl\u2010Neuberg\u2010Str. 1 30625 Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weikang","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Trauma Surgery Hannover Medical School  Carl\u2010Neuberg\u2010Str. 1 30625 Hannover Germany"},{"name":"Department of Spine Surgery Yu Huang Ding Hospital  Yu Dong Str. 20 Yan Tai 264000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessa","family":"Wagner","sequence":"additional","affiliation":[{"name":"Ulm University Medical Center Department of Trauma Hand, Plastic and Reconstructive Surgery Translational and Experimental Trauma Research  Helmholtz Str. 16 89081 Ulm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasmin Maria","family":"B\u00fclow","sequence":"additional","affiliation":[{"name":"Ulm University Medical Center Department of Trauma Hand, Plastic and Reconstructive Surgery Translational and Experimental Trauma Research  Helmholtz Str. 16 89081 Ulm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Borna","family":"Relja","sequence":"additional","affiliation":[{"name":"Ulm University Medical Center Department of Trauma Hand, Plastic and Reconstructive Surgery Translational and Experimental Trauma Research  Helmholtz Str. 16 89081 Ulm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5256-3732","authenticated-orcid":false,"given":"Katrin","family":"Bundkirchen","sequence":"additional","affiliation":[{"name":"Department of Trauma Surgery Hannover Medical School  Carl\u2010Neuberg\u2010Str. 1 30625 Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,20]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40001-021-00594-8"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13017-020-00330-3"},{"key":"e_1_2_9_3_1","unstructured":"World Health Organization (2024).WHO Mortality Database. 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