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These processes are influenced by a broad range of entwined events facilitated by chemical, electrical, and mechanical signals. The progress of each process is inherently linked to phenotypic changes in cells. Currently, the primary means of demonstrating morphological changes rely on measurements of neurite outgrowth and axon length. However, conventional techniques for monitoring these processes often require extensive preparation to enable manual or semi\u2010automated measurements. Here, a label\u2010free and non\u2010invasive approach is employed for monitoring neuronal differentiation and degeneration using quantitative phase imaging (QPI). Operating on unlabeled specimens and offering little to no phototoxicity and photobleaching, QPI delivers quantitative maps of optical path length delays that provide an objective measure of cellular morphology and dynamics. This approach enables the visualization and quantification of axon length and other physical properties of dorsal root ganglion (DRG) neuronal cells, allowing greater understanding of neuronal responses to stimuli simulating CIPN conditions. This research paves new avenues for the development of more effective strategies in the clinical management of neurotoxicity.<\/jats:p>","DOI":"10.1002\/adbi.202400020","type":"journal-article","created":{"date-parts":[[2024,3,28]],"date-time":"2024-03-28T23:24:03Z","timestamp":1711668243000},"source":"Crossref","is-referenced-by-count":3,"title":["Label\u2010Free Visualization and Morphological Profiling of Neuronal Differentiation and Axonal Degeneration through Quantitative Phase Imaging"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5689-1445","authenticated-orcid":false,"given":"Jeong Hee","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Mechanical Engineering Johns Hopkins University  Baltimore MD 21218 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aysel","family":"Cetinkaya\u2010Fisgin","sequence":"additional","affiliation":[{"name":"Department of Neurology Neuromuscular Division Johns Hopkins University School of Medicine  Baltimore MD 21205 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noah","family":"Zahn","sequence":"additional","affiliation":[{"name":"Department Chemical &amp; Biomolecular Engineering Johns Hopkins University  Baltimore MD 21218 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4789-9558","authenticated-orcid":false,"given":"Mehmet Can","family":"Sari","sequence":"additional","affiliation":[{"name":"Department of Neurology Neuromuscular Division Johns Hopkins University School of Medicine  Baltimore MD 21205 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1215-3373","authenticated-orcid":false,"given":"Ahmet","family":"Hoke","sequence":"additional","affiliation":[{"name":"Department of Neurology Neuromuscular Division Johns Hopkins University School of Medicine  Baltimore MD 21205 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0800-0825","authenticated-orcid":false,"given":"Ishan","family":"Barman","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering Johns Hopkins University  Baltimore MD 21218 USA"},{"name":"Department of Oncology The Russell H. Morgan Department of Radiology and Radiological Science Johns Hopkins University School of Medicine  Baltimore MD 21205 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,28]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1111\/ner.12028"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.2001.86.4.1773"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2017.02.040"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.24943"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxics3020198"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxics9100229"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2021.09.004"},{"key":"e_1_2_7_8_1","first-page":"3276","volume":"65","author":"Carozzi V. 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