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However, its potential nephrotoxic effects remain concerning. The mechanism underlying TP\u2010induced nephrotoxicity is inadequately elucidated, particularly at single\u2010cell resolution. Hence, single\u2010cell RNA sequencing (scRNA\u2010seq) of kidney tissues from control and TP\u2010treated mice is performed to generate a thorough description of the renal cell atlas upon TP treatment. Heterogeneous responses of nephron epithelial cells are observed after TP exposure, attributing differential susceptibility of cell subtypes to excessive reactive oxygen species and increased inflammatory responses. Moreover, TP disrupts vascular function by activating endothelial cell immunity and damaging fibroblasts. Severe immune cell damage\u00a0and the activation of pro\u2010inflammatory Macro_C1 cells are also observed with TP treatment. Additionally, ligand\u2010receptor crosstalk analysis reveals that the SPP1 (osteopontin) signaling pathway targeting Macro_C1 cells is triggered by TP treatment, which may promote the infiltration of Macro_C1 cells to exacerbate renal toxicity. Overall, this study provides comprehensive information on the transcriptomic profiles and cellular composition of TP\u2010associated nephrotoxicity at single\u2010cell resolution, which can strengthen the understanding of the pathogenesis of TP\u2010induced nephrotoxicity and provide valuable clues for the discovery of new therapeutic targets to ameliorate TP\u2010associated nephrotoxicity.<\/jats:p>","DOI":"10.1002\/adbi.202400120","type":"journal-article","created":{"date-parts":[[2024,6,12]],"date-time":"2024-06-12T05:16:20Z","timestamp":1718169380000},"source":"Crossref","is-referenced-by-count":4,"title":["A Single\u2010Cell Transcriptome Profiling of Triptolide\u2010Induced Nephrotoxicity in Mice"],"prefix":"10.1002","volume":"8","author":[{"given":"Jiangpeng","family":"Wu","sequence":"first","affiliation":[{"name":"School of Chinese Materia Medica Tianjin University of Traditional Chinese Medicine  Tianjin 301617 China"},{"name":"Department of Urology and Shenzhen Clinical 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