{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T02:44:53Z","timestamp":1784861093880,"version":"3.55.0"},"reference-count":43,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,5,21]],"date-time":"2024-05-21T00:00:00Z","timestamp":1716249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AG070145"],"award-info":[{"award-number":["AG070145"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DK119066"],"award-info":[{"award-number":["DK119066"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Advanced Biology"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Aging is associated with a decline in cardiac function. Exercise has been shown to effectively reduce the risks of cardiovascular diseases. Here whether a combination of endurance and resistance exercises can improve cardiac function in aged mice during late life is investigated. Through transcriptome analysis, several signaling pathways activated in the hearts of 22\u2010month\u2010old mice after combined exercise, including cardiac muscle contraction, mitophagy, and longevity regulation are identified. Combined exercise training mitigated age\u2010associated pathological cardiac hypertrophy, reduced oxidative stress, cardiac senescence, and enhanced cardiac function. Upstream stimulatory factor 2 (Usf2) is upregulated in the aged mouse hearts with combined exercise compared to sedentary mice. In the human cardiomyocytes senescent model, overexpression of Usf2 led to anti\u2010senescence effects, while knockdown of Usf2 exacerbated cellular senescence. The results suggest that a combination of endurance and resistance exercises, such as swimming and resistance running, can mitigate age\u2010related pathological cardiac remodeling and cardiac dysfunction in late life. These cardioprotective effects are likely due to the activation of Usf2 and its anti\u2010senescence effect. Therefore, Usf2 can potentially be a novel therapeutic target for mitigating age\u2010related cardiac dysfunction.<\/jats:p>","DOI":"10.1002\/adbi.202400137","type":"journal-article","created":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T02:49:35Z","timestamp":1716346175000},"source":"Crossref","is-referenced-by-count":4,"title":["Combined Endurance and Resistance Exercise Mitigates Age\u2010Associated Cardiac Dysfunction"],"prefix":"10.1002","volume":"9","author":[{"given":"Xiaowei","family":"Han","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences USF Health Taneja College of Pharmacy University of South Florida  Tampa FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Ashraf","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences USF Health Taneja College of Pharmacy University of South Florida  Tampa FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Shi","sequence":"additional","affiliation":[{"name":"Division of Rheumatology Department of Internal Medicine Vascular Biology Center Augusta University  Augusta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Augustine T.","family":"Nkembo","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences USF Health Taneja College of Pharmacy University of South Florida  Tampa FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srinivas M.","family":"Tipparaju","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences USF Health Taneja College of Pharmacy University of South Florida  Tampa FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6465-2599","authenticated-orcid":false,"given":"Wanling","family":"Xuan","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences USF Health Taneja College of Pharmacy University of South Florida  Tampa FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,21]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.55513"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.119.315889"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.109.861005"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2008.04.034"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ehj.2003.09.026"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-8703(92)90932-L"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2011.4179"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0929-6646(10)60116-7"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01236.2007"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.110.973784"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/00223980.2018.1470487"},{"key":"e_1_2_9_12_1","unstructured":"ACSM's Guidelines for Exercise Testing and Prescription2001."},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-201X.1998.00428.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1249\/01.MSS.0000147629.74832.52"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01013.2012"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2016.08.003"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2003001200018"},{"key":"e_1_2_9_18_1","first-page":"549","volume":"21","author":"Chang P.","year":"2020","journal-title":"Mol. Med. Rep."},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2022.1038385"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-021-02174-3"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1200250109"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2011.09.086"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jjcc.2021.03.004"},{"key":"e_1_2_9_24_1","first-page":"1","volume":"2014","author":"Chistiakov D. A.","year":"2014","journal-title":"Biomed Res. Int."},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2020.00018"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01608-z"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.114.302022"},{"key":"e_1_2_9_28_1","volume":"3","author":"Dai D. F.","year":"2023","journal-title":"J. Cardiovasc. Aging"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1249\/JES.0000000000000189"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2019.1704117"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/4532592"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.217349"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2020.101750"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apcsb.2023.03.001"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/function\/zqac027"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI158447"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells8070686"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvab161"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.14336\/AD.2021.0927"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jjcc.2019.05.002"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2020.00280"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.2337\/db21-0536"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/g3journal\/jkad091"}],"container-title":["Advanced Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adbi.202400137","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T16:18:50Z","timestamp":1759421930000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adbi.202400137"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,21]]},"references-count":43,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["10.1002\/adbi.202400137"],"URL":"https:\/\/doi.org\/10.1002\/adbi.202400137","archive":["Portico"],"relation":{},"ISSN":["2701-0198","2701-0198"],"issn-type":[{"value":"2701-0198","type":"print"},{"value":"2701-0198","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,21]]},"article-number":"2400137"}}