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The mechanisms of QGY and N\u2010QGY in the treatment of OP are probed from the perspective of osteoclast\u2010osteoblast balance. Thirty Sprague\u2010Dawley rats are randomly divided into N\u2010QGY group, QGY group, and Control group. Beyond control rats that orally took normal saline, other rats are orally administered with isometric N\u2010QGY or QGY twice every day for 3 days. The drug\u2010containing serum and control serum are prepared and their effects on osteoclast\u2010derived exosome secretion are determined by bicinchoninic acid assay (BCA), nanoparticle tracking analysis, and Western blot. GW4869 and Interleukin\u20101\u03b2 (IL\u20101\u03b2) are adopted as the exosome inhibitor and inducer, respectively. Exosome uptake, cell counting kit\u20108, alkaline phosphatase (ALP) staining, alizarin red staining, enzyme\u2010linked immunosorbent assay, quantitative real\u2010time polymerase chain reaction, and Western blot are performed to examine the effects of altered osteoclast exosome content on osteogenic differentiation of mesenchymal stem cells (MSCs). N\u2010QGY, QGY, and GW4869 inhibit osteoclast\u2010derived exosome secretion and exosome uptake by MSCs, whereas IL\u20101\u03b2 exerted the opposite effects (<jats:italic>p\u00a0<\/jats:italic>&lt;\u00a00.05). Different from IL\u20101\u03b2, N\u2010QGY, QGY, and GW4869 partially elevated MSC viability, osteocalcin secretion, ALP, RUNX Family Transcription Factor 2 (RUNX2) and Osteopontin (OPN) expressions, and calcium deposition in the osteoclast\u2010MSCs coculture system (<jats:italic>p\u00a0<\/jats:italic>&lt;\u00a00.05). Mechanically, osteoclasts increased Notum protein level but decreased \u03b2\u2010catenin level, which is enhanced by IL\u20101\u03b2 but is reversed by GW4869, QGY, and N\u2010QGY (<jats:italic>p\u00a0<\/jats:italic>&lt;\u00a00.05). And the effect of N\u2010QGY is more conspicuous than that of QGY (<jats:italic>P<\/jats:italic>&lt;0.05). N\u2010QGY\u2010containing serum inhibits exosome levels in osteoclasts, thereby enhancing osteogenic differentiation of MSCs via inhibition of Notum protein and promotion of \u03b2\u2010catenin protein.<\/jats:p>","DOI":"10.1002\/adbi.202400166","type":"journal-article","created":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T15:58:07Z","timestamp":1719503887000},"source":"Crossref","is-referenced-by-count":2,"title":["New\u2010QiangGuYin\u2010Containing Serum Inhibits Osteoclast\u2010Derived Exosome Secretion and Down\u2010Regulates Notum to Promote Osteoblast Differentiation"],"prefix":"10.1002","volume":"9","author":[{"given":"Shilong","family":"Meng","sequence":"first","affiliation":[{"name":"The Second Clinical School Zhejiang Chinese Medical University  Zhejiang Hangzhou 310053 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xu","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Second Clinical School Zhejiang Chinese Medical University  Zhejiang Hangzhou 310053 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Yu","sequence":"additional","affiliation":[{"name":"The Second Clinical School Zhejiang Chinese Medical University  Zhejiang Hangzhou 310053 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minghao","family":"Tong","sequence":"additional","affiliation":[{"name":"The Second Clinical School Zhejiang Chinese Medical University  Zhejiang Hangzhou 310053 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yifeng","family":"Yuan","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital Zhejiang Chinese Medical University  Zhejiang Hangzhou 310005 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanguang","family":"Cao","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital Zhejiang Chinese Medical University  Zhejiang Hangzhou 310005 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Xianju Branch of the Second Affiliated Hospital Zhejiang Chinese Medical University  Zhejiang, Taizhou 317300 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaolin","family":"Shi","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital Zhejiang Chinese Medical University  Zhejiang Hangzhou 310005 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7109-3174","authenticated-orcid":false,"given":"Kang","family":"Liu","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital Zhejiang Chinese Medical University  Zhejiang Hangzhou 310005 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,6,27]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.3803\/EnM.2021.301"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)62349-5"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.7861\/clinmedicine.14-2-187"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.11622\/smedj.2021036"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-8587(17)30188-2"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)02646-5"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/gscm.0000000000000009"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.911326"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.102376"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2011.09.030"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0254-6272(08)60049-7"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/6080597"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.203848"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu14173604"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2022.114122"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aau6977"},{"key":"e_1_2_9_17_1","first-page":"1","volume":"2021","author":"Hu Y.","year":"2021","journal-title":"Oxid Med. 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