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Fluorescent proteins are promising candidates as photoactive materials due to their high fluorescence quantum yield and absorption extinction coefficients in aqueous media. However, developing artificial bioinspired photosynthetic systems requires a detailed understanding of molecular interactions and energy transfer mechanisms in the required operating conditions. Here, the supramolecular self\u2010assembly and photophysical properties of fluorescent proteins complexed with organic dyes are investigated in aqueous media. Supercharged mGreenLantern protein, mutated to have a charge of +22, is complexed together with anionic zinc phthalocyanines having 4 or 16 carboxylate groups. The structural characterization reveals a strong electrostatic interaction between the moieties, accompanied by partial conformational distortion of the protein structure, yet without compromising the mGreenLantern chromophore integrity as suggested by the lack of emission features related to the neutral form of the chromophore. The self\u2010assembled biohybrid shows a total quenching of protein fluorescence, in favor of an energy transfer process from the protein to the phthalocyanine, as demonstrated by fluorescence lifetime and ultrafast transient absorption measurements. These results provide insight into the rich photophysics of fluorescent protein\u2013dye complexes, anticipating their applicability as water\u2010based photoactive materials.<\/jats:p>","DOI":"10.1002\/adbi.202400308","type":"journal-article","created":{"date-parts":[[2024,10,16]],"date-time":"2024-10-16T03:14:37Z","timestamp":1729048477000},"source":"Crossref","is-referenced-by-count":1,"title":["Elucidating the Supramolecular Interaction of Positively Supercharged Fluorescent Protein with Anionic Phthalocyanines"],"prefix":"10.1002","volume":"9","author":[{"given":"Sharon","family":"Saarinen","sequence":"first","affiliation":[{"name":"Department of Bioproducts and Biosystems Aalto University  Aalto 00076 Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramsha","family":"Khan","sequence":"additional","affiliation":[{"name":"Faculty of Engineering and Natural Sciences Tampere University  Korkeakoulunkatu 8 Tampere 33720 Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Patrian","sequence":"additional","affiliation":[{"name":"Technical University of Munich Campus Straubing for Biotechnology and Sustainability Chair of Biogenic Functional Materials  Schulgasse, 22 94315 Straubing Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Pablo","family":"Fuenzalida\u2010Werner","sequence":"additional","affiliation":[{"name":"Technical University of Munich Campus Straubing for Biotechnology and Sustainability Chair of Biogenic Functional Materials  Schulgasse, 22 94315 Straubing Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rub\u00e9n D.","family":"Costa","sequence":"additional","affiliation":[{"name":"Technical University of Munich Campus Straubing for Biotechnology and Sustainability Chair of Biogenic Functional Materials  Schulgasse, 22 94315 Straubing Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petr","family":"Zimcik","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy in Hradec Kralove Charles University  Ak. Heyrovskeho 1203 Hradec Kralove 50003 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronika","family":"Novakova","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy in Hradec Kralove Charles University  Ak. Heyrovskeho 1203 Hradec Kralove 50003 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tero\u2010Petri","family":"Ruoko","sequence":"additional","affiliation":[{"name":"Faculty of Engineering and Natural Sciences Tampere University  Korkeakoulunkatu 8 Tampere 33720 Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolai V.","family":"Tkachenko","sequence":"additional","affiliation":[{"name":"Faculty of Engineering and Natural Sciences Tampere University  Korkeakoulunkatu 8 Tampere 33720 Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Anaya\u2010Plaza","sequence":"additional","affiliation":[{"name":"Department of Bioproducts and Biosystems Aalto University  Aalto 00076 Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8282-2379","authenticated-orcid":false,"given":"Mauri A.","family":"Kostiainen","sequence":"additional","affiliation":[{"name":"Department of Bioproducts and Biosystems Aalto University  Aalto 00076 Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,16]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.244.4900.35"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3CS60405E"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1039\/B802262N"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsif.2012.0984"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1555"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201603118"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41557-018-0172-y"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41557-020-00580-3"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202006014"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.202300709"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201912654"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201711433"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp014643s"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nphoton.2011.99"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201502349"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201601792"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1088\/0957-4484\/27\/45\/45LT01"},{"key":"e_1_2_8_18_1","volume":"20","author":"Nizamoglu S.","year":"2016","journal-title":"SD\u00dc fen bilim. enst. 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