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Isotelekin in in vitro studies, inhibited the production of Th\u20101 cytokines Interleukin\u20106 (IL\u20106), Tumour necrosis factor (TNF\u2010\u03b1), and Interferon\u2010gamma (INF\u2010\u03b3),\u00a0and increased Th\u20102 cytokines Interleukin\u201010 (IL\u201010). Whereas it inhibited the nitrites and reactive oxygen species (ROS) production by mitigating the effect of LPS significantly. In vivo immunomodulatory activity in Delayed\u2010type hypersensitivity (DTH) and Hemagglutinating antibody (HA), Isotelekin suppressed the cellular as well as humoral immunity in immune\u2010affected and SRBC\u2010sensitized mice. Isotelekin decreased the phagocytic responses against carbon particles and plaque\u2010forming mainly IgG (Immunoglobulin G) production. Additionally, Isotelekin showed immunosuppressive potential through the evaluation of splenocytes, allograft acceptance, and haematological parameters. Molecular studies, including western blot analysis and immunocytochemistry, revealed that Isotelekin reduced the expression of iNOS (Inducible nitric oxide synthase), COX\u20102 (Cyclo\u2010Oxygenase 2), and p\u2010IkB\u03b1 (Phospho I\u2010kappa\u2010B\u2010alpha), and significantly inhibited the nuclear translocation of NF\u2010\u03baB\/p65. Based on these results, Isotelekin at 10 \u00b5<jats:sc>m<\/jats:sc> in in vitro and at 30\u00a0mg\u00a0kg<jats:sup>\u22121<\/jats:sup> in in vivo demonstrated strong anti\u2010inflammatory and immunosuppressive therapeutic potential.<\/jats:p>","DOI":"10.1002\/adbi.202400386","type":"journal-article","created":{"date-parts":[[2024,10,16]],"date-time":"2024-10-16T03:13:47Z","timestamp":1729048427000},"source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of Anti\u2010Inflammatory and Immunosuppressant Potential of Isotelekin in Lipopolysaccharide (LPS) Stimulated Macrophage (RAW 264.7) and Sheep Red Blood Cells (SRBC) Sensitized Murine Models"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-1656-033X","authenticated-orcid":false,"given":"Irfan","family":"Qasam","sequence":"first","affiliation":[{"name":"CSIR\u2010 Indian Institute of Integrative Medicine  Canal Road Jammu 180001 India"},{"name":"Mutagenicity Laboratory Pharmacology Division CSIR\u2010 Indian Institute of Integrative Medicine  Canal Road Jammu 18000 India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad 201002 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shah","family":"Nawaz","sequence":"additional","affiliation":[{"name":"CSIR\u2010 Indian Institute of Integrative Medicine  Canal Road Jammu 180001 India"},{"name":"Mutagenicity Laboratory Pharmacology Division CSIR\u2010 Indian Institute of Integrative Medicine  Canal Road Jammu 18000 India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad 201002 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hema","family":"Kumari","sequence":"additional","affiliation":[{"name":"CSIR\u2010 Indian Institute of Integrative Medicine  Canal Road Jammu 180001 India"},{"name":"Mutagenicity Laboratory Pharmacology Division CSIR\u2010 Indian Institute of Integrative Medicine  Canal Road Jammu 18000 India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad 201002 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Narendra","family":"Chauhan","sequence":"additional","affiliation":[{"name":"CSIR\u2010 Indian Institute of Integrative Medicine  Canal Road Jammu 180001 India"},{"name":"Mutagenicity Laboratory Pharmacology Division CSIR\u2010 Indian Institute of Integrative Medicine  Canal Road Jammu 18000 India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad 201002 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yedukondalu","family":"Nalli","sequence":"additional","affiliation":[{"name":"CSIR\u2010 Indian Institute of Integrative Medicine  Canal Road Jammu 180001 India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad 201002 India"},{"name":"Natural Product Chemistry Division CSIR\u2010Indian Institute of Integrative Medicine  Canal Road Jammu 180001 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Govind","family":"Yadav","sequence":"additional","affiliation":[{"name":"CSIR\u2010 Indian Institute of Integrative Medicine  Canal Road Jammu 180001 India"},{"name":"Mutagenicity Laboratory Pharmacology Division CSIR\u2010 Indian Institute of Integrative Medicine  Canal Road Jammu 18000 India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad 201002 India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,10,16]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1002\/fft2.129"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/healthcare11131946"},{"key":"e_1_2_10_3_1","first-page":"628","volume":"6","author":"Rezaei N.","year":"2023","journal-title":"Elsevier Sci."},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10787-023-01265-2"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/life13102105"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2024.103874"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/jpm13071078"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-19-9501-9_11"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00044-019-02337-1"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S432279"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2023.117127"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2022.155978"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules23123093"},{"key":"e_1_2_10_14_1","first-page":"2","volume":"8","author":"Deva K. 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