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To enhance their regenerative properties, ASCs can be chemically stimulated using various in vitro protocols. However, unsatisfactory results persist, partly due to the relatively costly long\u2010term methods. Furthermore, current culturing techniques often rely on the use of xenogenic fetal bovine serum that can be immunogenic, limiting clinical translations. To facilitate clinical translation of ASCs\u2010derived therapeutics, the effect of different stimulation protocols on human ASCs cultured in a xeno\u2010free medium (PRIME\u2010XV MSC Expansion XSFM) is investigated. The xeno\u2010free medium was supplemented with stimulants (forskolin (FSK), basic fibroblast growth factor, platelet\u2010derived growth factor\u2010AA, neuregulin\u20101) in combinations or individually. Stimulation for 72 h in FSK alone, or together with the growth factors, enhanced the production of urokinase plasminogen activator (uPA), a serine protease involved in tissue remodeling processes. Conditioned medium derived from stimulated ASCs enhanced in vitro angiogenesis and endothelial cells migration. This study shows that pro\u2010angiogenic responses in human ASCs can be enhanced with a defined short stimulation protocol using a xeno\u2010free medium. The protocol, using readily available manufacturing cell therapy grade molecules, may boost the regenerative properties of ASCs secretome which could enhance their efficacy in clinical treatments.<\/jats:p>","DOI":"10.1002\/adbi.202400466","type":"journal-article","created":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T14:44:58Z","timestamp":1752072298000},"source":"Crossref","is-referenced-by-count":0,"title":["Forskolin Enhances Urokinase Plasminogen Activator Secretion and Angiogenic Activity of Xeno\u2010Free Cultures of Human Adipose Tissue\u2010Derived Stem Cells"],"prefix":"10.1002","volume":"9","author":[{"given":"Maria Vittoria","family":"Giraudo","sequence":"first","affiliation":[{"name":"Department of Medical and Translational Biology Ume\u00e5 University  Ume\u00e5 90187 Sweden"},{"name":"Department of Diagnostics and Intervention Hand and Plastic Surgery Ume\u00e5 University  Ume\u00e5 901 87 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne Therese","family":"Lauvrud","sequence":"additional","affiliation":[{"name":"Department of Medical and Translational Biology Ume\u00e5 University  Ume\u00e5 90187 Sweden"},{"name":"Department of Diagnostics and Intervention Hand and Plastic Surgery Ume\u00e5 University  Ume\u00e5 901 87 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca","family":"Wiberg","sequence":"additional","affiliation":[{"name":"Department of Medical and Translational Biology Ume\u00e5 University  Ume\u00e5 90187 Sweden"},{"name":"Department of Diagnostics and Intervention Hand and Plastic Surgery Ume\u00e5 University  Ume\u00e5 901 87 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Brohlin","sequence":"additional","affiliation":[{"name":"Department of Medical and Translational Biology Ume\u00e5 University  Ume\u00e5 90187 Sweden"},{"name":"Department of Clinical Microbiology Ume\u00e5 University  Ume\u00e5 901 87 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gustav","family":"Andersson","sequence":"additional","affiliation":[{"name":"Department of Medical and Translational Biology Ume\u00e5 University  Ume\u00e5 90187 Sweden"},{"name":"Department of Diagnostics and Intervention Hand and Plastic Surgery Ume\u00e5 University  Ume\u00e5 901 87 Sweden"},{"name":"Wallenberg Centre for Molecular Medicine Ume\u00e5 University  Ume\u00e5 901 87 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2596-5936","authenticated-orcid":false,"given":"Paul J","family":"Kingham","sequence":"additional","affiliation":[{"name":"Department of Medical and Translational Biology Ume\u00e5 University  Ume\u00e5 90187 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,9]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5411.143"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mcb.2022.04.011"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e02-02-0105"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjps.2019.06.025"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.111.243147"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2008.03.006"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-019-1213-1"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-023-03381-w"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1089\/wound.2017.0775"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amsu.2021.102482"},{"key":"e_1_2_10_11_1","first-page":"429","volume":"26","author":"Nguyen T. 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