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Consequently, inhibiting MG activation and reducing the inflammatory response may be key strategies for the clinical treatment of stroke and neurodegenerative diseases. Edaravone (EDA), a potent anti\u2010inflammatory and antioxidant, is currently used in the clinical treatment of IS; however, its anti\u2010inflammatory mechanisms remain inadequately understood. To address this, network pharmacology (NP) analysis is employed to identify the phosphatidylinositol 3\u2010kinase\/protein kinase B (PI3K\/Akt) signaling pathway as a potential mediator of the inflammatory response triggered by activated microglia following EDA treatment. In vitro oxygen\u2010glucose deprivation (OGD) is used to induce BV\u20102 MG activation, and an in vivo middle cerebral artery occlusion (MCAO) mouse model is established. Western blot and immunofluorescence staining are used to detect changes in the phosphorylation levels of pathway\u2010related proteins and the expression of inflammatory factors. Additionally, the PI3K pathway inhibitor LY294002 and a PI3K overexpression plasmid are introduced to further analyze the expression changes of these markers. The results suggest that EDA may alleviate the inflammatory response mediated by activated MG through the PI3K\/Akt signaling pathway.<\/jats:p>","DOI":"10.1002\/adbi.202400501","type":"journal-article","created":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T11:21:41Z","timestamp":1744111301000},"source":"Crossref","is-referenced-by-count":3,"title":["Edaravone Alleviates BV\u20102 Microglia\u2010Mediated Neuroinflammation Through the PI3K\/AKT\/ NF\u2010\u03baB Pathway"],"prefix":"10.1002","volume":"9","author":[{"given":"Li","family":"Yang","sequence":"first","affiliation":[{"name":"School of basic medical science Kunming Medical University  Kunming 650500 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9215-7238","authenticated-orcid":false,"given":"Zhaoda","family":"Duan","sequence":"additional","affiliation":[{"name":"School of basic medical science Kunming Medical University  Kunming 650500 China"},{"name":"Institute of biomedical engineering Kunming Medical Univesity  Kunming 650500 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongyao","family":"Xu","sequence":"additional","affiliation":[{"name":"School of basic medical science Kunming Medical University  Kunming 650500 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingqi","family":"Peng","sequence":"additional","affiliation":[{"name":"School of basic medical science Kunming Medical University  Kunming 650500 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuke","family":"Wu","sequence":"additional","affiliation":[{"name":"First school of clinical medicine Kunming Medical University  Kunming 650500 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujia","family":"Yang","sequence":"additional","affiliation":[{"name":"School of basic medical science Kunming Medical University  Kunming 650500 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Yin","sequence":"additional","affiliation":[{"name":"School of basic medical science Kunming Medical University  Kunming 650500 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanxi","family":"Fang","sequence":"additional","affiliation":[{"name":"First school of clinical medicine Kunming Medical University  Kunming 650500 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Yan","sequence":"additional","affiliation":[{"name":"Institute of biomedical engineering Kunming Medical Univesity  Kunming 650500 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6349-4103","authenticated-orcid":false,"given":"Chunyun","family":"Wu","sequence":"additional","affiliation":[{"name":"School of basic medical science Kunming Medical University  Kunming 650500 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,8]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11011-023-01337-w"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.120.033272"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12883-017-1007-y"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.14715\/cmb\/2023.69.13.32"},{"key":"e_1_2_10_5_1","volume":"11","author":"Wang W.","year":"2016","journal-title":"PLoS One"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12868-015-0219-6"},{"key":"e_1_2_10_7_1","author":"Kashbour M.","year":"2025","journal-title":"Naunyn\u2010Schmiedeberg's Arch. 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