{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T00:50:21Z","timestamp":1759452621335,"version":"build-2065373602"},"reference-count":29,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T00:00:00Z","timestamp":1740700800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Advanced Biology"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The approval of JAK inhibitors, represented by baricitinib, for the treatment of alopecia areata (AA) has ushered in a new era. However, their excessive immunosuppressive effects have also raised numerous concerns. Therefore, the development of JAK inhibitors with different selectivities may enhance drug safety while maintaining therapeutic efficacy. CS12192, developed by our team, is a selective JAK3 inhibitor that also partially inhibits JAK1 and TBK1. In this study, we evaluate the effectiveness of CS12192 and baricitinib in a C3H\/HeJ skin\u2010transplanted AA mouse model and conduct in\u2010depth analysis of the similarities and differences in the mechanisms of the two compounds using mass cytometry (CyTOF) and RNA\u2010seq technology. The results show that CS12192 could reverse hair growth inhibition in AA mice in a dose\u2010dependent manner, with the high\u2010dose group exhibiting comparable effectiveness to baricitinib but with better safety. Further research revealed that both compounds significantly reduced the infiltration of immune cells, particularly CD8<jats:sup>+<\/jats:sup> T cells, in the skin of AA animals. CyTOF and RNA\u2010seq analysis revealed the mechanistic similarities between CS12192 and baricitinib in regulating AA\u2010related immune cells and signaling pathways. In conclusion, CS12192, as a novel selective JAK inhibitor, holds great potential for the treatment of AA.<\/jats:p>","DOI":"10.1002\/adbi.202400769","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T09:52:34Z","timestamp":1741686754000},"source":"Crossref","is-referenced-by-count":0,"title":["CS12192 Reverses Alopecia Areata by Selectively Targeting JAK3\/JAK1\/TBK1"],"prefix":"10.1002","volume":"9","author":[{"given":"Heping","family":"Jin","sequence":"first","affiliation":[{"name":"Chengdu Chipscreen Pharmaceutical Corp., Ltd.  Chengdu Sichuan P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zongyang","family":"Li","sequence":"additional","affiliation":[{"name":"Chengdu Chipscreen Pharmaceutical Corp., Ltd.  Chengdu Sichuan P. R. 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