{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T21:08:20Z","timestamp":1788556100959,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T00:00:00Z","timestamp":1758672000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T00:00:00Z","timestamp":1758672000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Advanced Biology"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Small nucleolar RNAs (snoRNAs)\u201460\u2013300 nucleotide non\u2010coding RNAs are associated with adverse clinical outcomes in cancer patients. However, information on the role of snoRNAs and associated small nuclear ribonucleoprotein (snoRNPs) in prostate cancer (PCa) remains scarce. Here, the contribution of the snoRNP U3 snoRNA\u2010interacting protein 2 (\n                    <jats:italic>RRP9<\/jats:italic>\n                    ) in PCa pathogenesis is investigated. A combination of three different shRNAs is employed to knockdown\n                    <jats:italic>RRP9<\/jats:italic>\n                    in the PCa cell lines DU\u2010145 and PC\u20103. Cell proliferation is evaluated by seeding cells into a 96\u2010well plates and monitoring daily. Cell migration is evaluated by scratch and Transwell assays. FLAG\u2010\n                    <jats:italic>RRP9<\/jats:italic>\n                    pull\u2010down, MALDI\u2010TOF\/TOF, and co\u2010immunoprecipitation assays are conducted to identify\n                    <jats:italic>RRP9<\/jats:italic>\n                    binding partners in DU\u2010145 cells. In vitro cell proliferation and migration, as well as in vivo tumor growth, are suppressed following\n                    <jats:italic>RRP9<\/jats:italic>\n                    knockdown. Pull\u2010down and MALDI\u2010TOF\/TOF analyses identified five putative\n                    <jats:italic>RRP9<\/jats:italic>\n                    binding partners, and co\u2010immunoprecipitation validated that\n                    <jats:italic>RRP9<\/jats:italic>\n                    interacts with the scaffolding hub protein Sequestome\u20101 (SQSTM1, p62). Interestingly,\n                    <jats:italic>SQSTM1<\/jats:italic>\n                    overexpression rescued the anti\u2010growth\/migration effects of\n                    <jats:italic>RRP9<\/jats:italic>\n                    knockdown. This study unveiled a novel oncogenic role for the\n                    <jats:italic>RRP9<\/jats:italic>\n                    \u2010SQSTM1 axis in PCa cells.\n                    <jats:italic>RRP9<\/jats:italic>\n                    is a snoRNP that binds to SQSTM1, thereby promoting PCa cell proliferation and migration. Targeting the\n                    <jats:italic>RRP9<\/jats:italic>\n                    \u2010SQSTM1 axis may be a viable therapeutic strategy for PCa.\n                  <\/jats:p>","DOI":"10.1002\/adbi.202500182","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T06:19:52Z","timestamp":1758781192000},"source":"Crossref","is-referenced-by-count":1,"title":["snoRNP\n                    <i>RRP9<\/i>\n                    Promotes Prostate Cancer Cell Proliferation and Migration via SQSTM1"],"prefix":"10.1002","volume":"9","author":[{"given":"Butang","family":"Li","sequence":"first","affiliation":[{"name":"Department of Urology Ningbo Hangzhou Bay Hospital  Zhejiang 315336 China"},{"name":"Department of Urology Jiading District Central Hospital  Shanghai 201800 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihui","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Urology Ningbo Hangzhou Bay Hospital  Zhejiang 315336 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Urology Jiading District Central Hospital  Shanghai 201800 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Urology Ningbo Hangzhou Bay Hospital  Zhejiang 315336 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4574-2529","authenticated-orcid":false,"given":"Xiaorong","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Urology Ningbo Hangzhou Bay Hospital  Zhejiang 315336 China"},{"name":"Department of Urology Renji Hospital School of Medicine Shanghai Jiao Tong University  Shanghai 200127 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenfei","family":"Chi","sequence":"additional","affiliation":[{"name":"Department of Urology Ningbo Hangzhou Bay Hospital  Zhejiang 315336 China"},{"name":"Department of Urology Jiading District Central Hospital  Shanghai 201800 China"},{"name":"Department of Urology Renji Hospital School of Medicine Shanghai Jiao Tong University  Shanghai 200127 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahua","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Urology Ningbo Hangzhou Bay Hospital  Zhejiang 315336 China"},{"name":"Department of Urology Renji Hospital School of Medicine Shanghai Jiao Tong University  Shanghai 200127 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,24]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21492"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/CU9.0000000000000120"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/CU9.0000000000000173"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/CU9.0000000000000145"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2021.4708"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2015.00002"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2939"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2017.10.070"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2013.89"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3563"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.13889"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2019.00788"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa066"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2018100801"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13062-024-00578-8"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.22737"},{"key":"e_1_2_7_17_1","first-page":"1","volume":"8","author":"Lima A. 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