{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T04:08:32Z","timestamp":1781928512929,"version":"3.54.5"},"reference-count":46,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T00:00:00Z","timestamp":1762905600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T00:00:00Z","timestamp":1762905600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP24K12981"],"award-info":[{"award-number":["JP24K12981"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Advanced Biology"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This study investigates the effects of ion release and pH elevation from a biodegradable metallic magnesium (Mg) \u201330 calcium (Ca) coating on osteogenesis using osteoblast\u2010like cells. The coating, formed on titanium (Ti) via magnetron sputtering, has previously been shown to enhance osteogenesis by promoting calcite formation on the Ti surface upon degradation in vitro study. However, the individual and combined roles of released Mg\n                    <jats:sup>2+<\/jats:sup>\n                    , Ca\n                    <jats:sup>2+<\/jats:sup>\n                    , and pH elevation remain unclear. To clarify these effects, culture media supplemented with Mg\n                    <jats:sup>2+<\/jats:sup>\n                    and Ca\n                    <jats:sup>2+<\/jats:sup>\n                    salts are prepared. Mg\n                    <jats:sup>2+<\/jats:sup>\n                    at 4\u20135\u00a0m\n                    <jats:sc>m<\/jats:sc>\n                    promotes early alkaline phosphatase (ALP) activity compared to the 0.9\u00a0m\n                    <jats:sc>m<\/jats:sc>\n                    control, without affecting proliferation but suppressing mineralization. Ca\n                    <jats:sup>2+<\/jats:sup>\n                    at 2.3\u20133\u00a0m\n                    <jats:sc>m<\/jats:sc>\n                    enhances ALP activity without affecting proliferation or mineralization compared to the 1.3\u20132.2\u00a0m\n                    <jats:sc>m<\/jats:sc>\n                    control. When both ions coexist, proliferation, ALP activity, and mineralization are enhanced compared to Mg\n                    <jats:sup>2+<\/jats:sup>\n                    alone, suggesting a synergistic effect. Furthermore, the elevated pH resulting from the Mg\u201330Ca extract more effectively promotes proliferation, accelerates the peak of ALP activity, and supports mineralization than ions co\u2010supplementation. These findings indicate that Mg\u201330Ca coatings enhance osteogenesis through both ion release and pH elevation, providing new insight into the osteogenic potential of biodegradable metallic coatings.\n                  <\/jats:p>","DOI":"10.1002\/adbi.202500401","type":"journal-article","created":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T12:32:24Z","timestamp":1762950744000},"source":"Crossref","is-referenced-by-count":5,"title":["Osteogenic Effects of Ion Released from Biodegradable Metallic Magnesium and Calcium Coating"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-2279-8195","authenticated-orcid":false,"given":"Risa","family":"Miyake","sequence":"first","affiliation":[{"name":"Department of Regenerative and Reconstructive Dental Medicine Graduate School of Medical and Dental Sciences Institute of Science Tokyo  1\u20105\u201045 Yushima Bunkyo\u2010ku Tokyo 113\u20108549 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0364-8721","authenticated-orcid":false,"given":"Masaya","family":"Shimabukuro","sequence":"additional","affiliation":[{"name":"Department of Inorganic Biomaterials Laboratory for Biomaterials and Bioengineering Institute of Integrated Research Institute of Science Tokyo  2\u20103\u201010, Kanda\u2010Surugadai Chiyoda\u2010ku Tokyo 101\u20100062 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiko","family":"Terauchi","sequence":"additional","affiliation":[{"name":"Department of Regenerative and Reconstructive Dental Medicine Graduate School of Medical and Dental Sciences Institute of Science Tokyo  1\u20105\u201045 Yushima Bunkyo\u2010ku Tokyo 113\u20108549 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eriko","family":"Marukawa","sequence":"additional","affiliation":[{"name":"Department of Regenerative and Reconstructive Dental Medicine Graduate School of Medical and Dental Sciences Institute of Science Tokyo  1\u20105\u201045 Yushima Bunkyo\u2010ku Tokyo 113\u20108549 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masakazu","family":"Kawashita","sequence":"additional","affiliation":[{"name":"Department of Inorganic Biomaterials Laboratory for Biomaterials and Bioengineering Institute of Integrated Research Institute of Science Tokyo  2\u20103\u201010, Kanda\u2010Surugadai Chiyoda\u2010ku Tokyo 101\u20100062 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,12]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.3390\/jfb8040044"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnsc.2014.08.014"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jma.2021.06.024"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2014.02.002"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202000800"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom11040506"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2018.01.049"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2014.08.011"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.12.021"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13577-012-0041-8"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0921-5093(97)00806-X"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/10667857.2016.1212780"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3109\/17453678608994393"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2006.06.025"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12179"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S255486"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.4c01341"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.langmuir.5c01746"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.3c00860"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1248\/yakushi.126.651"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2015.04.011"},{"key":"e_1_2_9_23_1","doi-asserted-by":"crossref","unstructured":"W. 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