{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T17:59:32Z","timestamp":1785347972074,"version":"3.55.0"},"reference-count":68,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100006668","name":"Fondo para la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006668","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Advanced Biology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Biomaterials have been developed with a great potential in tissue engineering. Hydrogels, especially those based on poly\u2010\n                    <jats:italic>N<\/jats:italic>\n                    \u2010isopropylacrylamide (PNIPAM), have innate mechanical and physicochemical similarities with the native extracellular matrix. We have previously reported the in Vitro compatibility of PNIPAM and its copolymers with a varied number of cell lines, displaying good proliferation rates and bioadhesive capacity. However, the in Vivo biocompatibility assessment is necessary to evaluate. In this work, biocompatibility and immunological acceptance of macroporous hydrogels based on PNIPAM and its co\u2010polymer PNIPAM\u2010co\u20103%APTA (3\u2010acrylamidopropyl chloride)trimethyl\u2010ammonium) were analyzed using a Wistar female rat model. Hydrogels were synthesized by free radical polymerization and an in Vitro assessment was performed in RAW 264.7 murine macrophages. Then, polymers were implanted subcutaneously and 3\u2010months afterward, grafts were retrieved along with blood and organ samples for haematological, blood chemistry and histological analysis. Cytotoxicity and nitric oxide production did not show variability for the hydrogels. No alterations in the healing process and complete blood count were observed as well as in systemic, liver or kidney toxicity. Histological analysis revealed graft\u00b4s integration with the surrounding tissue, without evidence of fibrous capsule or immune cell infiltration. Therefore, these hydrogels are promising biocompatible scaffolds to be used as tissue\u2010engineered constructs.\n                  <\/jats:p>","DOI":"10.1002\/adbi.202500529","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T17:30:43Z","timestamp":1784223043000},"source":"Crossref","is-referenced-by-count":0,"title":["Macroporous Poly\u2010\n                    <i>N<\/i>\n                    \u2010Isopropylacrylamide and Cationic Co\u2010Polymer Hydrogels: Long\u2010Term Subcutaneous Implant Biocompatibility"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3662-2376","authenticated-orcid":false,"given":"Virginia","family":"Capella","sequence":"first","affiliation":[{"name":"Chemistry Department Faculty of Exact Physical Chemical and Natural Sciences, Institute of Research in Energy Technologies and Advanced Materials (IITEMA) National University of Rio Cuarto (UNRC) \u2013 National Council of Scientific and Technical Research (CONICET)  R\u00edo Cuarto Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jimena","family":"Arri","sequence":"additional","affiliation":[{"name":"Animal Clinic Department Faculty of Agronomy and Veterinary Medicine National University of Rio Cuarto (UNRC)  R\u00edo Cuarto Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana C.","family":"Liaudat","sequence":"additional","affiliation":[{"name":"Molecular Biology Department Faculty of Exact Physical Chemical and Natural Sciences Institute of Environmental Biotechnology and Health (INBIAS) National University of Rio Cuarto (UNRC) \u2013 National Council of Scientific and Technical Research (CONICET)  R\u00edo Cuarto Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nahir D.","family":"Panozzo Lacunza","sequence":"additional","affiliation":[{"name":"Chemistry Department Faculty of Exact Physical Chemical and Natural Sciences, Institute of Research in Energy Technologies and Advanced Materials (IITEMA) National University of Rio Cuarto (UNRC) \u2013 National Council of Scientific and Technical Research (CONICET)  R\u00edo Cuarto Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bianca A.","family":"Opizzo Balza","sequence":"additional","affiliation":[{"name":"Molecular Biology Department Faculty of Exact Physical Chemical and Natural Sciences Institute of Environmental Biotechnology and Health (INBIAS) National University of Rio Cuarto (UNRC) \u2013 National Council of Scientific and Technical Research (CONICET)  R\u00edo Cuarto Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rocio B.","family":"Bonino","sequence":"additional","affiliation":[{"name":"Molecular Biology Department Faculty of Exact Physical Chemical and Natural Sciences Institute of Environmental Biotechnology and Health (INBIAS) National University of Rio Cuarto (UNRC) \u2013 National Council of Scientific and Technical Research (CONICET)  R\u00edo Cuarto Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gricelda D.","family":"Morilla","sequence":"additional","affiliation":[{"name":"Animal Clinic Department Faculty of Agronomy and Veterinary Medicine National University of Rio Cuarto (UNRC)  R\u00edo Cuarto Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matias","family":"Caverzan","sequence":"additional","affiliation":[{"name":"Molecular Biology Department Faculty of Exact Physical Chemical and Natural Sciences Institute of Environmental Biotechnology and Health (INBIAS) National University of Rio Cuarto (UNRC) \u2013 National Council of Scientific and Technical Research (CONICET)  R\u00edo Cuarto Argentina"},{"name":"Animal Pathology Department. Faculty of Agronomy and Veterinary Medicine National University of Rio Cuarto (UNRC)  R\u00edo Cuarto Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cesar A.","family":"Barbero","sequence":"additional","affiliation":[{"name":"Chemistry Department Faculty of Exact Physical Chemical and Natural Sciences, Institute of Research in Energy Technologies and Advanced Materials (IITEMA) National University of Rio Cuarto (UNRC) \u2013 National Council of Scientific and Technical Research (CONICET)  R\u00edo Cuarto Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Bosch","sequence":"additional","affiliation":[{"name":"Molecular Biology Department Faculty of Exact Physical Chemical and Natural Sciences Institute of Environmental Biotechnology and Health (INBIAS) National University of Rio Cuarto (UNRC) \u2013 National Council of Scientific and Technical Research (CONICET)  R\u00edo Cuarto Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia R.","family":"Rivarola","sequence":"additional","affiliation":[{"name":"Chemistry Department Faculty of Exact Physical Chemical and Natural Sciences, Institute of Research in Energy Technologies and Advanced Materials (IITEMA) National University of Rio Cuarto (UNRC) \u2013 National Council of Scientific and Technical Research (CONICET)  R\u00edo Cuarto Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Molecular Biology Department Faculty of Exact Physical Chemical and Natural Sciences Institute of Environmental Biotechnology and Health (INBIAS) National University of Rio Cuarto (UNRC) \u2013 National Council of Scientific and Technical Research (CONICET)  R\u00edo Cuarto Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41578\u2010018\u20100023\u2010x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-100803-4.00005-X"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2018.0325"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2017.01.056"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms161025392"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9TB01539F"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41578\u2010020\u20100209\u2010x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2021.04.017"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2018.00499"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781003340485-14"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2009.09.009"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201303233"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2017.05.006"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41570\u2010022\u201000420\u20107"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijsolstr.2024.112920"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202008235"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.langmuir.2c02253"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2MA00527A"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp0690603"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2016.09.081"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2004.09.008"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reactfunctpolym.2015.10.011"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748\u20106041\/7\/3\/035003"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2017.07.032"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9RA08162C"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2019.e01474"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/admi.202201162"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.34577"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201545818"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2007.11.004"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.35958"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5RA01679G"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40942\u2010016\u20100048\u20105"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.24813"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022\u20101759(83)90303\u20104"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378\u20104274(85)90046\u20103"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1051\/vetres\/2009014"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.141.7.2407"},{"key":"e_1_2_9_40_1","unstructured":"D. R.Askeland P. P.Fulay andW. J.Wright The Science and Engineering of Materials 6th ed. Cengage Learning 2011."},{"key":"e_1_2_9_41_1","unstructured":"I. A. G.De SeguraandJ. B.dela V\u00edbora inCiencia y Tecnolog\u00eda en Experimentaci\u00f3n y Protecci\u00f3n Animal (Eds:J. M.Z\u00faniga J. M.Orellana Muriana) Editorial Universidad de Alcal\u00e1 Spain2016."},{"key":"e_1_2_9_42_1","volume-title":"Wintrobe's Clinical Hematology","author":"Wintrobe M. M.","year":"2008"},{"key":"e_1_2_9_43_1","volume-title":"Histopathology: Methods and Protocols","author":"Feldman A. T.","year":"2014"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot073411"},{"key":"e_1_2_9_45_1","volume-title":"InfoStat Versi\u00f3n","author":"Di Rienzo J. A.","year":"2018"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.polymer.2011.11.037"},{"issue":"3","key":"e_1_2_9_47_1","article-title":"Valores de Refer\u00eancia Hematol\u00f3gicos e Bioqu\u00edmicos de Ratos (Rattus novergicus linhagem Wistar) Provenientes do Biot\u00e9rio da Universidade Tiradentes","volume":"10","author":"Lima C. M.","year":"2014","journal-title":"Scientia plena"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.36062\/ijah.60.1.2021.58\u201065"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20100030\u20101248720"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033\u2010023\u201008666\u20100"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mpaic.2021.05.010"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201700927"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmrt.2021.05.009"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1039\/C0SM00617C"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reactfunctpolym.2025.106560"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.34940"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1021\/ab5001063"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mpsur.2017.06.004"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.3390\/gels3040036"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.3390\/gels8070454"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2026.103377"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym13234071"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2017.06.019"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2023.122234"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-4160-6189-2.00073-1"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1002\/cld.562"},{"key":"e_1_2_9_67_1","volume-title":"Interpretaci\u00f3n y Diagn\u00f3stico","author":"Meyer D. J.","year":"2000"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2020.02.041"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10047\u2010004\u20100265\u20109"}],"container-title":["Advanced Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adbi.202500529","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adbi.202500529","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adbi.202500529","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T17:14:11Z","timestamp":1785345251000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adbi.202500529"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":68,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/adbi.202500529"],"URL":"https:\/\/doi.org\/10.1002\/adbi.202500529","archive":["Portico"],"relation":{},"ISSN":["2701-0198","2701-0198"],"issn-type":[{"value":"2701-0198","type":"print"},{"value":"2701-0198","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7]]},"article-number":"e00529"}}