{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T04:19:38Z","timestamp":1787804378710,"version":"build-2784847793"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Advanced Biology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Cerebral ischemia\u2010reperfusion injury (CIRI) is a severe neurological condition. LncRNA\u2010XIST is closely associated with CIRI; however, its underlying mechanism remains unclear. A middle cerebral artery occlusion\/reperfusion (MCAO\/R) rat model and an oxygen\u2010glucose deprivation\/reoxygenation (OGD\/R) model in neuroblastoma cells (SH\u2010SY5Y) were established. The expression of lncRNA\u2010XIST, microRNA\u2010532\u20105p, and PTEN was detected by qPCR. Binding sites between lncRNA\u2010XIST and microRNA\u2010532\u20105p, and between microRNA\u2010532\u20105p and PTEN, were predicted using bioinformatic approaches and validated by dual\u2010luciferase assays. Cell viability, apoptosis, and oxidative stress were assessed by CCK\u20108 assay, flow cytometry\/Western blot, and MDA\/SOD assay kits, respectively. LncRNA\u2010XIST and PTEN were highly expressed in the MCAO\/R rat brain tissues and the OGD\/R cell model, whereas microRNA\u2010532\u20105p was expressed at low levels. Knockdown of lncRNA\u2010XIST attenuated OGD\/R\u2010induced cellular injury. Furthermore, lncRNA\u2010XIST was found to target and inhibit microRNA\u2010532\u20105p expression, while microRNA\u2010532\u20105p in turn targeted and suppressed PTEN. The downregulation of PTEN expression and decrease in the apoptotic rate and oxidative stress levels induced by lncRNA\u2010XIST knockdown in the OGD\/R cell model were reversed by microRNA\u2010532\u20105p knockdown. LncRNA\u2010XIST promotes the development and progression of CIRI via the microRNA\u2010532\u20105p\/PTEN axis, offering potential therapeutic targets for CIRI.<\/jats:p>","DOI":"10.1002\/adbi.202500593","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T04:18:12Z","timestamp":1785903492000},"source":"Crossref","is-referenced-by-count":0,"title":["LncRNA\u2010XIST Competitively Binds to MicroRNA\u2010532\u20105p to Regulate PTEN Expression, Exacerbating Cerebral Ischemia\u2010Reperfusion Injury"],"prefix":"10.1002","volume":"10","author":[{"given":"Xudong","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Neurology Jiaxing Second Hospital  Jiaxing Zhejiang China"},{"name":"JiaXing Key Laboratory of Fundamental and Clinical Research on Cerebral Protection in Acute Ischemic Cerebrovascular Disease (A)  Jiaxing Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luhan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Neurology Jiaxing Second Hospital  Jiaxing Zhejiang China"},{"name":"JiaXing Key Laboratory of Fundamental and Clinical Research on Cerebral Protection in Acute Ischemic Cerebrovascular Disease (A)  Jiaxing Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liqi","family":"Qian","sequence":"additional","affiliation":[{"name":"Department of Neurology Jiaxing Second Hospital  Jiaxing Zhejiang China"},{"name":"JiaXing Key Laboratory of Fundamental and Clinical Research on Cerebral Protection in Acute Ischemic Cerebrovascular Disease (A)  Jiaxing Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoling","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Neurology Jiaxing Second Hospital  Jiaxing Zhejiang China"},{"name":"JiaXing Key Laboratory of Fundamental and Clinical Research on Cerebral Protection in Acute Ischemic Cerebrovascular Disease (A)  Jiaxing Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanping","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurology Jiaxing Second Hospital  Jiaxing Zhejiang China"},{"name":"JiaXing Key Laboratory of Fundamental and Clinical Research on Cerebral Protection in Acute Ischemic Cerebrovascular Disease (A)  Jiaxing Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-4930-2052","authenticated-orcid":false,"given":"Bo","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Neurology Jiaxing Second Hospital  Jiaxing Zhejiang China"},{"name":"JiaXing Key Laboratory of Fundamental and Clinical Research on Cerebral Protection in Acute Ischemic Cerebrovascular Disease (A)  Jiaxing Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,4]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/17474930211065917"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/neurintsurg-2018-014568"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.4103\/bc.bc_9_24"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a036780"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a036194"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnves.2025.100046"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2024.115023"},{"key":"e_1_2_10_9_1","first-page":"6035","article-title":"SETD5\u2010AS1 stimulates Neuron Death in Stroke via Promoting PTEN Expression","volume":"22","author":"Miao S. 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