{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:38:37Z","timestamp":1787312317758,"version":"build-2736575974"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"am","delay-in-days":26,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["EEC 1941543"],"award-info":[{"award-number":["EEC 1941543"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014895","name":"Open Philanthropy Project","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014895","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Advanced Biology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Mosquitoes remain a significant global health concern due to their role in transmitting deadly diseases including malaria, dengue, and yellow fever. Recent advances in genetic modification technologies have enabled researchers to better study disease transmission and is an emerging approach for vector control. However, a major bottleneck is the need for continuous rearing of each new strain, an intensive process requiring substantial manual effort and space with inherent risks of cross\u2010contamination, genetic drift, and catastrophic loss. Cryopreservation offers a potential solution, where strains could be stored indefinitely and revived on an as\u2010needed basis. Here, we report a cryopreservation protocol that results in the recovery of viable\n                    <jats:italic>Anopheles gambiae<\/jats:italic>\n                    larvae. After thawing, 82% of larvae showed signs of viability, including wriggling and mouth brush movements. Approximately 15% further exhibited coordinated swimming behavior. Although post\u2010thaw survival was limited to 24 h, this study provides the first evidence that mosquito larvae can survive cryopreservation, representing a key milestone toward long\u2010term storage of mosquito lines.\n                  <\/jats:p>","DOI":"10.1002\/adbi.202500633","type":"journal-article","created":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T13:52:02Z","timestamp":1769521922000},"source":"Crossref","is-referenced-by-count":2,"title":["Cryopreservation of\n                    <i>Anopheles gambiae<\/i>\n                    Larvae"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0362-4946","authenticated-orcid":false,"given":"Purva","family":"Joshi","sequence":"first","affiliation":[{"name":"Center for Engineering in Medicine &amp; Surgery Department of Surgery Massachusetts General Hospital Harvard Medical School, and Shriners Children's  Boston USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachary","family":"Chau","sequence":"additional","affiliation":[{"name":"Center for Engineering in Medicine &amp; Surgery Department of Surgery Massachusetts General Hospital Harvard Medical School, and Shriners Children's  Boston USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abby","family":"Callahan\u2010Muller","sequence":"additional","affiliation":[{"name":"Center for Engineering in Medicine &amp; Surgery Department of Surgery Massachusetts General Hospital Harvard Medical School, and Shriners Children's  Boston USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent O.","family":"Nyasembe","sequence":"additional","affiliation":[{"name":"CDC Foundation Assigned to Division of Parasitic Diseases and Malaria, Entomology Branch Centers for Disease Control and Prevention (CDC)  Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kala","family":"Acha","sequence":"additional","affiliation":[{"name":"CDC Foundation Assigned to Division of Parasitic Diseases and Malaria, Entomology Branch Centers for Disease Control and Prevention (CDC)  Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen M.","family":"Dotson","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention  Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmet","family":"Toner","sequence":"additional","affiliation":[{"name":"Center for Engineering in Medicine &amp; Surgery Department of Surgery Massachusetts General Hospital Harvard Medical School, and Shriners Children's  Boston USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3077-2135","authenticated-orcid":false,"given":"Rebecca D.","family":"Sandlin","sequence":"additional","affiliation":[{"name":"Center for Engineering in Medicine &amp; Surgery Department of Surgery Massachusetts General Hospital Harvard Medical School, and Shriners Children's  Boston USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,27]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201643233"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010ento\u2010010814\u2010020828"},{"key":"e_1_2_10_4_1","unstructured":"Vector\u2010borne diseases https:\/\/www.who.int\/news\u2010room\/fact\u2010sheets\/detail\/vector\u2010borne\u2010diseases(2024)."},{"key":"e_1_2_10_5_1","first-page":"131","volume-title":"Reviews of Environmental Contamination and Toxicology","author":"Wolmarans N. 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