{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T04:19:37Z","timestamp":1787804377444,"version":"build-2784847793"},"reference-count":128,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Advanced Biology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>In this Review, we summarize recent conceptual and technological advances in single\u2010cell glycomics, with a particular focus on emerging strategies that enable glycan\u2010resolved analysis of tumor\u2013immune interactions. We highlight single\u2010cell glycan and RNA sequencing (scGR\u2010seq), a methodology that converts glycan information into amplifiable nucleic acid signals, enabling simultaneous profiling of transcriptomes and glycomes at the single\u2010cell level. In parallel, we introduce GlycoChat, a computational framework designed to systematically infer and visualize glycan\u2013lectin interaction networks within the TME. Integrative application of these approaches to pancreatic ductal adenocarcinoma (PDAC) reveals extensive glycan remodeling during epithelial\u2013mesenchymal transition (EMT) and demonstrates that basal\u2010like PDAC cells selectively reinforce interactions with lectin receptors expressed on innate immune cells, particularly tumor\u2010associated macrophages. These EMT\u2010driven glycan\u2013lectin circuits are proposed to transmit immunosuppressive signals within the TME and show a strong association with clinical outcomes. Collectively, these advances highlight glycans as active mediators, rather than solely passive tumor markers of immune regulation, and provide a conceptual framework for understanding and potentially exploiting glycan\u2010mediated immune regulation in cancer.<\/jats:p>","DOI":"10.1002\/adbi.70152","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T15:16:11Z","timestamp":1787670971000},"source":"Crossref","is-referenced-by-count":0,"title":["Single\u2010Cell Glycomics of the Pancreatic Tumor Microenvironment: Technologies, Glyco\u2010Immune Checkpoints, and Tumor\u2013Immune Communication"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-6126-6259","authenticated-orcid":false,"given":"Dinh Xuan Tuan","family":"Anh","sequence":"first","affiliation":[{"name":"Cellular and Molecular Biotechnology Research Institute National Institute of Advanced Industrial Science and Technology (AIST)  Tsukuba Ibaraki Japan"},{"name":"Ph.D. Program in Human Biology School of Integrative and Global Majors University of Tsukuba  Tsukuba Ibaraki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3006-1659","authenticated-orcid":false,"given":"Hiroaki","family":"Tateno","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Biotechnology Research Institute National Institute of Advanced Industrial Science and Technology (AIST)  Tsukuba Ibaraki Japan"},{"name":"Institute of Medicine University of Tsukuba  Tsukuba Ibaraki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,25]]},"reference":[{"key":"e_1_2_19_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2022.102395"},{"key":"e_1_2_19_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.32.2.227\u2010237.1936"},{"key":"e_1_2_19_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304\u20104165(68)90218\u20103"},{"key":"e_1_2_19_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378\u20104347(00)80822\u20101"},{"key":"e_1_2_19_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076\u20106879(06)15019\u20103"},{"key":"e_1_2_19_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.357"},{"key":"e_1_2_19_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003\u20102697(89)90213\u20103"},{"key":"e_1_2_19_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmr.300030507"},{"key":"e_1_2_19_10_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8OB01664J"},{"key":"e_1_2_19_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-1779-3_4"},{"key":"e_1_2_19_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscentsci.2c00215"},{"key":"e_1_2_19_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.1c04779"},{"key":"e_1_2_19_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202011015"},{"key":"e_1_2_19_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0407902101"},{"key":"e_1_2_19_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt735"},{"key":"e_1_2_19_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0302\u2010275"},{"key":"e_1_2_19_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2019.05.017"},{"key":"e_1_2_19_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200400403"},{"key":"e_1_2_19_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth803"},{"key":"e_1_2_19_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5315.1125"},{"key":"e_1_2_19_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076\u20106879(06)15015\u20106"},{"key":"e_1_2_19_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio0605\u201013"},{"key":"e_1_2_19_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200900942"},{"key":"e_1_2_19_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2009206117"},{"key":"e_1_2_19_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1114356109"},{"key":"e_1_2_19_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpz1.104"},{"key":"e_1_2_19_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2021.102882"},{"key":"e_1_2_19_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abe3610"},{"key":"e_1_2_19_30_1","volume-title":"Essentials of Glycobiology","author":"Bertozzi C. R.","year":"2009"},{"key":"e_1_2_19_31_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3cs35419a"},{"key":"e_1_2_19_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-68186-2"},{"key":"e_1_2_19_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2020.11.087"},{"key":"e_1_2_19_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.104419"},{"key":"e_1_2_19_35_1","first-page":"67","volume-title":"Essentials of Glycobiology","author":"Rini J. M.","year":"2022"},{"key":"e_1_2_19_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadva.2024.100125"},{"key":"e_1_2_19_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576\u2010019\u20100093\u20107"},{"key":"e_1_2_19_38_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2025-012391"},{"key":"e_1_2_19_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202514735"},{"key":"e_1_2_19_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpz1.777"},{"key":"e_1_2_19_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/smtd.202301338"},{"key":"e_1_2_19_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2025.102631"},{"key":"e_1_2_19_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwaf006"},{"key":"e_1_2_19_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2024.09.010"},{"key":"e_1_2_19_45_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvn043"},{"key":"e_1_2_19_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2022.101179"},{"key":"e_1_2_19_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43705\u2010021\u201000084\u20102"},{"key":"e_1_2_19_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2025.11.005"},{"key":"e_1_2_19_49_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.3c02602"},{"key":"e_1_2_19_50_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.78870"},{"key":"e_1_2_19_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201047772\u2010w"},{"key":"e_1_2_19_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/nargab\/lqae169"},{"key":"e_1_2_19_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.105163"},{"key":"e_1_2_19_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadva.2025.100146"},{"key":"e_1_2_19_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010025\u201056390\u2010z"},{"key":"e_1_2_19_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2024.126056"},{"key":"e_1_2_19_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201073525\u2010y"},{"key":"e_1_2_19_58_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201014\u20100155"},{"key":"e_1_2_19_59_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2024.1386699"},{"key":"e_1_2_19_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575\u2010023\u201000840\u2010w"},{"key":"e_1_2_19_61_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2025.1569884"},{"key":"e_1_2_19_62_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159\u20108290.CD\u201022\u20100876"},{"key":"e_1_2_19_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571\u2010025\u201001077\u2010z"},{"key":"e_1_2_19_64_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046\u2010023\u201002935\u20103"},{"key":"e_1_2_19_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590\u2010025\u201002134\u20106"},{"key":"e_1_2_19_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cpt.2024.07.004"},{"key":"e_1_2_19_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201040850\u20105"},{"key":"e_1_2_19_68_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046\u2010025\u201003377\u20109"},{"key":"e_1_2_19_69_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cww086"},{"key":"e_1_2_19_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2016.09.013"},{"key":"e_1_2_19_71_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3982"},{"key":"e_1_2_19_72_1","doi-asserted-by":"publisher","DOI":"10.1158\/2326\u20106066.CIR\u201020\u20100264"},{"key":"e_1_2_19_73_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.202451318"},{"key":"e_1_2_19_74_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943\u2010025\u201002417\u20104"},{"key":"e_1_2_19_75_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21550-4"},{"key":"e_1_2_19_76_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.RA120.002256"},{"key":"e_1_2_19_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcpro.2021.100160"},{"key":"e_1_2_19_78_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535\u20107163.MCT\u201017\u20100232"},{"key":"e_1_2_19_79_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2017.05.012"},{"key":"e_1_2_19_80_1","doi-asserted-by":"publisher","DOI":"10.1155\/2024\/1529449"},{"key":"e_1_2_19_81_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2024.109037"},{"key":"e_1_2_19_82_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwad009"},{"key":"e_1_2_19_83_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.34362"},{"key":"e_1_2_19_84_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.15023"},{"key":"e_1_2_19_85_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2023-007805"},{"key":"e_1_2_19_86_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.00732"},{"key":"e_1_2_19_87_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2025.167900"},{"key":"e_1_2_19_88_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14572"},{"key":"e_1_2_19_89_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003\u2010021\u201002934\u20100"},{"key":"e_1_2_19_90_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010025\u201002884\u20106"},{"key":"e_1_2_19_91_1","doi-asserted-by":"publisher","DOI":"10.1080\/14712598.2021.1882989"},{"key":"e_1_2_19_92_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1388"},{"key":"e_1_2_19_93_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573\u2010020\u201000093\u20101"},{"key":"e_1_2_19_94_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI180282"},{"key":"e_1_2_19_95_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.14759"},{"key":"e_1_2_19_96_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom11020188"},{"key":"e_1_2_19_97_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad905"},{"key":"e_1_2_19_98_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2018.3"},{"key":"e_1_2_19_99_1","first-page":"375","volume-title":"Essentials of Glycobiology","author":"Taylor M. E.","year":"2022"},{"key":"e_1_2_19_100_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2024.107869"},{"key":"e_1_2_19_101_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-02404-1"},{"key":"e_1_2_19_102_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2016.00033"},{"key":"e_1_2_19_103_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines9070770"},{"key":"e_1_2_19_104_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43018\u2010026\u201001154\u2010x"},{"key":"e_1_2_19_105_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2025.07.013"},{"key":"e_1_2_19_106_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3552"},{"key":"e_1_2_19_107_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201025\u20100977"},{"key":"e_1_2_19_108_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms242115500"},{"key":"e_1_2_19_109_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2024.03.007"},{"key":"e_1_2_19_110_1","doi-asserted-by":"publisher","DOI":"10.1158\/2326\u20106066.CIR\u201021\u20101088"},{"key":"e_1_2_19_111_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-020-1186-7"},{"key":"e_1_2_19_112_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4314"},{"key":"e_1_2_19_113_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2026.01.020"},{"key":"e_1_2_19_114_1","doi-asserted-by":"publisher","DOI":"10.1021\/jasms.4c00494"},{"key":"e_1_2_19_115_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.3c05984"},{"key":"e_1_2_19_116_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590\u2010023\u201001678\u20109"},{"key":"e_1_2_19_117_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.08.019"},{"key":"e_1_2_19_118_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004784"},{"key":"e_1_2_19_119_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.8b00515"},{"key":"e_1_2_19_120_1","first-page":"653","volume-title":"Essentials of Glycobiology","author":"Rudd P.","year":"2015"},{"key":"e_1_2_19_121_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt829"},{"key":"e_1_2_19_122_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003\u2010024\u201007277\u20100"},{"key":"e_1_2_19_123_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2024.09.006"},{"key":"e_1_2_19_124_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.554311"},{"key":"e_1_2_19_125_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.251173"},{"key":"e_1_2_19_126_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010024\u201002314\u20106"},{"key":"e_1_2_19_127_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-026-73050-y"},{"key":"e_1_2_19_128_1","doi-asserted-by":"publisher","DOI":"10.1111\/imcb.70011"},{"key":"e_1_2_19_129_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.3c12487"}],"container-title":["Advanced Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adbi.70152","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adbi.70152","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adbi.70152","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T04:07:25Z","timestamp":1787803645000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adbi.70152"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":128,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/adbi.70152"],"URL":"https:\/\/doi.org\/10.1002\/adbi.70152","archive":["Portico"],"relation":{},"ISSN":["2701-0198","2701-0198"],"issn-type":[{"value":"2701-0198","type":"print"},{"value":"2701-0198","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"article-number":"e70152"}}