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Herein, a facile magnetic extrusion method is developed for preparing endosome\u2010derived vesicles, also known as exosome mimetics (EMs), which share the same biological origin and similar morphology, composition, and biofunctions with native exosomes. The high yield and consistency of this magnetic extrusion method help to overcome the manufacturing bottleneck in exosome research. Moreover, the proposed standardized multi\u2010step method readily facilitates the ammonium sulfate gradient approach to actively load chemodrugs such as doxorubicin into EMs. The engineered EMs developed and tested here exhibit comparable drug delivery properties as do native exosomes, and potently inhibit tumor growth by delivering doxorubicin in an orthotopic breast tumor model. These findings demonstrate that EMs can be prepared in a facile and scaled\u2010up manner as a promising biological nanomedicine for cancer drug delivery.<\/jats:p>","DOI":"10.1002\/adfm.202008326","type":"journal-article","created":{"date-parts":[[2021,1,20]],"date-time":"2021-01-20T10:16:05Z","timestamp":1611137765000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["A Facile Magnetic Extrusion Method for Preparing Endosome\u2010Derived Vesicles for Cancer Drug Delivery"],"prefix":"10.1002","volume":"31","author":[{"given":"Peng","family":"Guo","sequence":"first","affiliation":[{"name":"Vascular Biology Program Boston Children's Hospital 300 Longwood Avenue  Boston MA 02115 USA"},{"name":"Department of Surgery Boston Children's Hospital Harvard Medical School 300 Longwood Avenue  Boston MA 02115 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Busatto","sequence":"additional","affiliation":[{"name":"Vascular Biology Program Boston Children's Hospital 300 Longwood Avenue  Boston MA 02115 USA"},{"name":"Department of Surgery Boston Children's Hospital Harvard Medical School 300 Longwood Avenue  Boston MA 02115 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Huang","sequence":"additional","affiliation":[{"name":"Vascular Biology Program Boston Children's Hospital 300 Longwood Avenue  Boston MA 02115 USA"},{"name":"Department of Surgery Boston Children's Hospital Harvard Medical School 300 Longwood Avenue  Boston MA 02115 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9460-618X","authenticated-orcid":false,"given":"Golnaz","family":"Morad","sequence":"additional","affiliation":[{"name":"Vascular Biology Program Boston Children's Hospital 300 Longwood Avenue  Boston MA 02115 USA"},{"name":"Department of Surgery Boston Children's Hospital Harvard Medical School 300 Longwood Avenue  Boston MA 02115 USA"},{"name":"Graduate School of Arts and Sciences Harvard University 1350 Massachusetts Avenue  Cambridge MA 02138 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3108-0704","authenticated-orcid":false,"given":"Marsha A.","family":"Moses","sequence":"additional","affiliation":[{"name":"Vascular Biology Program Boston Children's Hospital 300 Longwood Avenue  Boston MA 02115 USA"},{"name":"Department of Surgery Boston Children's Hospital Harvard Medical School 300 Longwood Avenue  Boston MA 02115 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,1,20]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-016-0366-z"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-018-0036-9"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aav8521"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2019.1627164"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10544-019-0396-7"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2018.1560809"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2018.09.015"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells7120273"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2018.01.006"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2020.08.001"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2013.08.014"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.5b00364"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.37097"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-019-0485-1"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2016.06.024"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn402232g"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/nano10091816"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-67207-y"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-19628-z"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(90)82018-W"},{"key":"e_1_2_7_21_1","doi-asserted-by":"crossref","DOI":"10.1093\/oso\/9780199634958.001.0001","volume-title":"Subcellular Fractionation: A Practical Approach","author":"Graham J. 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