{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T00:22:53Z","timestamp":1778718173296,"version":"3.51.4"},"reference-count":19,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,2,12]],"date-time":"2014-02-12T00:00:00Z","timestamp":1392163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:p>Pulmonary immunization poses the unique challenge of balancing vaccine efficacy with minimizing inflammation in the respiratory tract. While previous studies have shown that mice immunized intranasally with F1\u2010V\u2010loaded polyanhydride nanoparticles are protected from a lethal challenge with <jats:italic>Yersinia pestis<\/jats:italic>, little is known about the initial interaction between the nanoparticles and immune cells following intranasal administration. Here, the deposition within the lung and internalization by phagocytic cells of polyanhydride nanovaccines encapsulating F1\u2010V are compared with that of soluble F1\u2010V alone or F1\u2010V adjuvanted with monophosphoryl lipid A (MPLA). Encapsulation of F1\u2010V into polyanhydride nanoparticles prolonged its presence while F1\u2010V administered with MPLA is undetectable within 48 h. The inflammation induced by the polyanhydride nanovaccine is mild compared with the marked inflammation induced by the MPLA\u2010adjuvanted F1\u2010V. Even though F1\u2010V delivered with saline is detected in the lung 48 h after administration, it is known that this regimen does not elicit a protective immune response. The prolonged F1\u2010V presence in the lung in concert with the mild inflammatory response provided by the nanovaccine provides new insights into the development of protective immune responses with a single intranasal dose.<\/jats:p>","DOI":"10.1002\/adhm.201300525","type":"journal-article","created":{"date-parts":[[2014,2,12]],"date-time":"2014-02-12T07:05:54Z","timestamp":1392188754000},"page":"1071-1077","source":"Crossref","is-referenced-by-count":24,"title":["Lung Deposition and Cellular Uptake Behavior of Pathogen\u2010Mimicking Nanovaccines in the First 48 Hours"],"prefix":"10.1002","volume":"3","author":[{"given":"Kathleen A.","family":"Ross","sequence":"first","affiliation":[{"name":"Department of Chemical and Biological Engineering Iowa State University  Ames IA 50011 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shannon L.","family":"Haughney","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering Iowa State University  Ames IA 50011 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Latrisha K.","family":"Petersen","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering Iowa State University  Ames IA 50011 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Boggiatto","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Preventive Medicine Iowa State University  Ames IA 50011 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"Wannemuehler","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Preventive Medicine Iowa State University  Ames IA 50011 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Balaji","family":"Narasimhan","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering Iowa State University  Ames IA 50011 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,2,12]]},"reference":[{"key":"e_1_2_6_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2008.09.005"},{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/1061186X.2010.523790"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.21493"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.09.033"},{"key":"e_1_2_6_5_1","first-page":"186","volume":"139","author":"Von Garnier C.","year":"2009","journal-title":"Swiss Med. 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