{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T08:06:57Z","timestamp":1789978017316,"version":"4.0.1"},"reference-count":62,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,12,16]],"date-time":"2014-12-16T00:00:00Z","timestamp":1418688000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:p>Porousbiodegradable polymer scaffolds are widely utilized for bone tissue engineering, but are not osteoconductive like calcium phosphate scaffolds. We combine indirect solid freeform fabrication (SFF), ex vivo gene therapy, with biomineral coating to compare the effect of biomineral coating on bone regeneration for Poly (<jats:sc>l<\/jats:sc>\u2010lactic acid) (PLLA) and Poly (\u03b5\u2010caprolactone) (PCL) scaffolds with the same porous architecture. Scanning electron microscope (SEM) and micro\u2010computed tomography (\u03bc\u2010CT) demonstrate PLLA and PCL scaffolds have the same porous architecture and are completely coated. All scaffolds are seeded with human gingival fibroblasts (HGF) transduced with adenovirus encoded with either bone morphogenetic protein 7 (BMP\u20107) or green fluorescent protein (GFP), and implanted into mice subcutaneously for 3 and 10 weeks. Only scaffolds with BMP\u20107 transduced HGFs show mineralized tissue formation. At 3 weeks some blood vessel\u2010like structures are observed in coated PLLA and PCL scaffolds, but there is no significant difference in bone ingrowth between the coated and uncoated scaffolds for either PLLA or PCL. At 10 weeks, however, coated scaffolds (both PLLA and PCL) have significantly more bone ingrowth than uncoated scaffolds, which have more fibrous tissue. Coated PLLA scaffolds have improved mechanical properties compared with uncoated PLLA scaffolds due to increased bone ingrowth.<\/jats:p>","DOI":"10.1002\/adhm.201400424","type":"journal-article","created":{"date-parts":[[2014,12,16]],"date-time":"2014-12-16T13:54:38Z","timestamp":1418738078000},"page":"621-632","source":"Crossref","is-referenced-by-count":37,"title":["Biomineral Coating Increases Bone Formation by Ex Vivo BMP\u20107 Gene Therapy in Rapid Prototyped Poly(<scp>l<\/scp>\u2010lactic acid) (PLLA) and Poly(\u03b5\u2010caprolactone) (PCL) Porous Scaffolds"],"prefix":"10.1002","volume":"4","author":[{"given":"Eiji","family":"Saito","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering 1101 Beal Ave. University of Michigan  Ann Arbor MI 48109\u20132099 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darilis","family":"Suarez\u2010Gonzalez","sequence":"additional","affiliation":[{"name":"Materials Science Program University of Wisconsin  Madison WI 53706 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William L.","family":"Murphy","sequence":"additional","affiliation":[{"name":"Materials Science Program University of Wisconsin  Madison WI 53706 USA"},{"name":"Department of Biomedical Engineering University of Wisconsin  Madison WI 53706 USA"},{"name":"Department of Orthopedics and Rehabilitation University of Wisconsin  Madison WI 53706 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott J.","family":"Hollister","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering 1101 Beal Ave. University of Michigan  Ann Arbor MI 48109\u20132099 USA"},{"name":"Department of Mechanical Engineering University of Michigan  Ann Arbor MI 48109\u20132125 USA"},{"name":"Department of Surgery University of Michigan  Ann Arbor MI 48109\u2013032 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,12,16]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1163\/156856201744489"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/BA20030109"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2004.05.005"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmat1421"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.132"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2010.06.012"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-010-4091-8"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm901260y"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.01.016"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(00)00101-0"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ppap.201000153"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcs.143"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2007.00078.x"},{"key":"e_1_2_7_14_1","first-page":"667","volume":"11","author":"Hutmacher D.","year":"1996","journal-title":"Int. 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