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In this study, the development of a NP formulation that can meet all these challenging requirements for targeted glioblastoma multiform (GBM) therapy is reported. This multifunctional NP is composed of a polyethylene glycol\u2010coated magnetic iron oxide NP conjugated with cyclodextrin and chlorotoxin (CTX) and loaded with fluorescein and paclitaxel (PTX) (IONP\u2010PTX\u2010CTX\u2010FL). The physicochemical properties of the IONP\u2010PTX\u2010CTX\u2010FL are characterized by transmission electron microscope, dynamic light scattering, and high\u2010performance liquid chromatography. The cellular uptake of NPs is studied using flow cytometry and confocal microscopy. Cell viability and apoptosis are assessed with the Alamar Blue viability assay and flow cytometry, respectively. The IONP\u2010PTX\u2010CTX\u2010FL had a uniform size of \u224844 nm and high stability in cell culture medium. Importantly, the presence of CTX on NPs enhanced the uptake of the NPs by GBM cells and improved the efficacy of PTX in killing both GBM and GBM drug\u2010resistant cells. The IONP\u2010PTX\u2010CTX\u2010FL demonstrated its great potential for brain cancer therapy and may also be used to deliver PTX to treat other cancers.<\/jats:p>","DOI":"10.1002\/adhm.201500034","type":"journal-article","created":{"date-parts":[[2015,3,11]],"date-time":"2015-03-11T12:25:46Z","timestamp":1426076746000},"page":"1236-1245","source":"Crossref","is-referenced-by-count":55,"title":["Stable and Efficient Paclitaxel Nanoparticles for Targeted Glioblastoma Therapy"],"prefix":"10.1002","volume":"4","author":[{"given":"Qingxin","family":"Mu","sequence":"first","affiliation":[{"name":"Department of Materials Science and Engineering University of Washington  Seattle WA 98195 USA"},{"name":"Clinical Research Division Fred Hutchinson Cancer Research Center  Seattle WA 98109 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mike","family":"Jeon","sequence":"additional","affiliation":[{"name":"Department of Materials Science and Engineering University of Washington  Seattle WA 98195 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng\u2010Hsuan","family":"Hsiao","sequence":"additional","affiliation":[{"name":"Department of Materials Science and Engineering National Chiao Tung University  Hsinchu City 300 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria K.","family":"Patton","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering University of Washington  Seattle WA 98195 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kui","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Materials Science and Engineering University of Washington  Seattle WA 98195 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver W.","family":"Press","sequence":"additional","affiliation":[{"name":"Clinical Research Division Fred Hutchinson Cancer Research Center  Seattle WA 98109 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miqin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Materials Science and Engineering University of Washington  Seattle WA 98195 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,11]]},"reference":[{"key":"e_1_2_6_1_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra0708126"},{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.20803"},{"key":"e_1_2_6_2_2","first-page":"3759","volume":"29","author":"Sharma S.","year":"2009","journal-title":"Anticancer Res."},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(00)00466-0"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2012.03.047"},{"key":"e_1_2_6_5_1","first-page":"445","volume":"6","author":"Tian X.\u2010H.","year":"2011","journal-title":"Int. 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