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To overcome these limitations, numerous cell\u2010based approaches for cartilage repair are being explored that aim to provide an appropriate microenvironment for chondrocyte maintenance and differentiation of multipotent mesenchymal stem cells (MSCs) toward the chondrogenic lineage. Articular cartilage is composed of highly organized collagen network that entails the tissue into four distinct zones and each zone into three different regions based on differences in matrix morphology and biochemistry. Current cartilage implants cannot establish the hierarchical tissue organization that seems critical for normal cartilage function. Therefore, in this study, a structured, multilayered collagen scaffold designed for the replacement of damaged cartilage is presented that allows repopulation by host cells and synthesis of a new natural matrix. By using the electrospinning method, the potential to engineer a scaffold consisting of two different collagen types is obtained. With the developed collagen scaffold, a five\u2010layered biomaterial is created that has the potency to induce the differentiation of human bone marrow derived MSCs toward the chondrogenic lineage.<\/jats:p>","DOI":"10.1002\/adhm.201600134","type":"journal-article","created":{"date-parts":[[2016,5,17]],"date-time":"2016-05-17T02:27:01Z","timestamp":1463452021000},"page":"2191-2198","source":"Crossref","is-referenced-by-count":34,"title":["Investigation of Migration and Differentiation of Human Mesenchymal Stem Cells on Five\u2010Layered Collagenous Electrospun Scaffold Mimicking Native Cartilage Structure"],"prefix":"10.1002","volume":"5","author":[{"given":"Jenny W.","family":"Reboredo","sequence":"first","affiliation":[{"name":"Department Tissue Engineering and Regenerative Medicine University Hospital W\u00fcrzburg  R\u00f6ntgenring 11 97070 W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Weigel","sequence":"additional","affiliation":[{"name":"Department Tissue Engineering and Regenerative Medicine University Hospital W\u00fcrzburg  R\u00f6ntgenring 11 97070 W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre","family":"Steinert","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, K\u00f6nig\u2010Ludwig\u2010Haus Orthopaedic Center for Musculoskeletal Research Julius\u2010Maximilians\u2010University W\u00fcrzburg  Brettreichstra\u00dfe 11 W\u00fcrzburg 97074 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Rackwitz","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, K\u00f6nig\u2010Ludwig\u2010Haus Orthopaedic Center for Musculoskeletal Research Julius\u2010Maximilians\u2010University W\u00fcrzburg  Brettreichstra\u00dfe 11 W\u00fcrzburg 97074 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maximilian","family":"Rudert","sequence":"additional","affiliation":[{"name":"Department Tissue Engineering and Regenerative Medicine University Hospital W\u00fcrzburg  R\u00f6ntgenring 11 97070 W\u00fcrzburg Germany"},{"name":"Department of Orthopedic Surgery, K\u00f6nig\u2010Ludwig\u2010Haus Orthopaedic Center for Musculoskeletal Research Julius\u2010Maximilians\u2010University W\u00fcrzburg  Brettreichstra\u00dfe 11 W\u00fcrzburg 97074 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heike","family":"Walles","sequence":"additional","affiliation":[{"name":"Department Tissue Engineering and Regenerative Medicine University Hospital W\u00fcrzburg  R\u00f6ntgenring 11 97070 W\u00fcrzburg Germany"},{"name":"Translational Center W\u00fcrzburg \u201cRegenerative Therapies in Oncology and Musculoskeletal Diseases\u201d W\u00fcrzburg Branch Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB  R\u00f6ntgenring 11 97070 W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,5,17]]},"reference":[{"key":"e_1_2_7_1_1","unstructured":"Orthoworld The Orthopaedic Industry Annual Report 2009."},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v025a18"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar2195"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(03)01157-8"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.13.9645"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9290(03)00110-6"},{"key":"e_1_2_7_7_1","first-page":"215","volume":"39","author":"van Osch G. 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