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Here, a bifunctional electrospun poly(\u03b5\u2010caprolactone) (PCL) scaffold with potential for synthetic vascular graft applications is presented. This scaffold incorporates two tethered peptides: the osteopontin\u2010derived peptide (Adh) on the \u201cluminal\u201d side and a heparin\u2010binding peptide (Hep) on the \u201cabluminal\u201d side. Additionally, the \u201cabluminal\u201d side of the scaffold is seeded with saphenous vein\u2010derived pericytes (SVPs) as a source of proangiogenic growth factors. The Adh peptide significantly increases endothelial cell adhesion, while the Hep peptide promotes accumulation of vascular endothelial growth factor secreted by SVPs. SVPs increase endothelial migration both in a transwell assay and a modified scratch assay performed on the PCL scaffold. Seeding of SVPs on the \u201cabluminal\u201d\/Hep side of the scaffold further increases endothelial cell density, indicating a combinatory effect of the peptides and pericytes. Finally, SVP\u2010seeded scaffolds are preserved by freezing in a xeno\u2010free medium, maintaining good cell viability and function. In conclusion, this engineered scaffold combines patient\u2010derived pericytes and spatially organized functionalities, which synergistically increase endothelial cell density and growth factor retention.<\/jats:p>","DOI":"10.1002\/adhm.201600699","type":"journal-article","created":{"date-parts":[[2016,10,26]],"date-time":"2016-10-26T07:33:07Z","timestamp":1477467187000},"page":"3046-3055","source":"Crossref","is-referenced-by-count":36,"title":["Pericyte Seeded Dual Peptide Scaffold with Improved Endothelialization for Vascular Graft Tissue Engineering"],"prefix":"10.1002","volume":"5","author":[{"given":"Paola","family":"Campagnolo","sequence":"first","affiliation":[{"name":"Department of Materials Department of Bioengineering and Institute of Biomedical Engineering Royal School of Mines Imperial College London  Prince Consort Rd SW7 2AZ London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam J.","family":"Gormley","sequence":"additional","affiliation":[{"name":"Department of Materials Department of Bioengineering and Institute of Biomedical Engineering Royal School of Mines Imperial College London  Prince Consort Rd SW7 2AZ London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lesley W.","family":"Chow","sequence":"additional","affiliation":[{"name":"Department of Materials Department of Bioengineering and Institute of Biomedical Engineering Royal School of Mines Imperial College London  Prince Consort Rd SW7 2AZ London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne G\u00e9raldine","family":"Guex","sequence":"additional","affiliation":[{"name":"Department of Materials Department of Bioengineering and Institute of Biomedical Engineering Royal School of Mines Imperial College London  Prince Consort Rd SW7 2AZ London UK"},{"name":"National Heart and Lung Institute Imperial College London  435 Du Cane Road W12 0NN London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paresh A.","family":"Parmar","sequence":"additional","affiliation":[{"name":"Department of Materials Department of Bioengineering and Institute of Biomedical Engineering Royal School of Mines Imperial College London  Prince Consort Rd SW7 2AZ London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer L.","family":"Puetzer","sequence":"additional","affiliation":[{"name":"Department of Materials Department of Bioengineering and Institute of Biomedical Engineering Royal School of Mines Imperial College London  Prince Consort Rd SW7 2AZ London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph A. 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