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It is vital to develop cost\u2010effective, highly sensitive and selective multitarget biosensing platforms that allow for both the detection and phenotyping. Here, a highly programmable, cost\u2010efficient and multianalyte sensing approach is reported that enables visual detection and differentiation of conserved oligonucleotide regions of all <jats:italic>Ebolavirus<\/jats:italic> subtypes known to infect human primates. This approach enables the detection of as little as 400 amols (24 \u00d7 10<jats:sup>6<\/jats:sup> molecules) of target sequences with the naked eye. Furthermore, the detection assay can be used to classify four virus biomarkers using a single nanoprobe template. This can be achieved by using different combinations of short single stranded initiator molecules, referred to as programming units, which also enable the simultaneous and rapid identification of the four biomarkers in 16 different combinations. The results of 16 \u00d7 5 array studies illustrate that the system is extremely selective with no false\u2010positive or false\u2010negative. Finally, the target strands in liquid biopsy mimics prepared from urine specimens are also able to be identified and classified.<\/jats:p>","DOI":"10.1002\/adhm.201600739","type":"journal-article","created":{"date-parts":[[2016,12,19]],"date-time":"2016-12-19T03:22:43Z","timestamp":1482117763000},"source":"Crossref","is-referenced-by-count":16,"title":["Rapid Visual Screening and Programmable Subtype Classification of Ebola Virus Biomarkers"],"prefix":"10.1002","volume":"6","author":[{"given":"Mustafa","family":"Balcioglu","sequence":"first","affiliation":[{"name":"Department of Chemistry University at Albany State University of New York  1400 Washington Avenue Albany NY 12222 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhit","family":"Rana","sequence":"additional","affiliation":[{"name":"Department of Chemistry University at Albany State University of New York  1400 Washington Avenue Albany NY 12222 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa Salih","family":"Hizir","sequence":"additional","affiliation":[{"name":"Department of Chemistry University at Albany State University of New York  1400 Washington Avenue Albany NY 12222 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil M.","family":"Robertson","sequence":"additional","affiliation":[{"name":"Department of Chemistry University at Albany State University of New York  1400 Washington Avenue Albany NY 12222 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kashfia","family":"Haque","sequence":"additional","affiliation":[{"name":"Department of Chemistry University at Albany State University of New York  1400 Washington Avenue Albany NY 12222 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmet V.","family":"Yigit","sequence":"additional","affiliation":[{"name":"Department of Chemistry University at Albany State University of New York  1400 Washington Avenue Albany NY 12222 USA"},{"name":"The RNA Institute University at Albany State University of New York  1400 Washington Avenue Albany NY 12222 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,12,19]]},"reference":[{"key":"e_1_2_6_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms9000"},{"key":"e_1_2_6_1_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1259657"},{"key":"e_1_2_6_1_3","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1404505"},{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature16038"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature16041"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.15-0229"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms9147"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2013.70"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1510824112"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1039\/C5LC00055F"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0126281"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.11.4125-4130.2001"},{"key":"e_1_2_6_7_1","first-page":"1481","volume":"371","year":"2014","journal-title":"W. 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