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Here for the first time it is reported the production of an organotypic cervical model featured by a scaffold\u2010free stromal tissue resembling the extracellular matrix (ECM) composition and organization of the native counterpart as well as a completely well\u2010differentiated epithelium. To reach this aim, human cervical microtissue precursors have been produced, characterized, and used as functional building units to fabricate a cell\u2010synthesized cervical stroma equivalent by means of a bottom\u2010up approach. Immunotypization, and molecular and morphological analyses reveal the extent of fundamental epithelial biomarkers and the presence of collagen and noncollagenous molecules, demonstrating that the natural tissue architecture and biological characteristics of cervical tissues are reproduced. The results of this study suggest that the bottom\u2010up technology used to produce these 3D human cervical equivalents provides a fully functional organotypic cervical model that may be used as a valuable tool to investigate the epithelial\u2010stromal interactions as well as for testing new therapeutics in vitro.<\/jats:p>","DOI":"10.1002\/adhm.201601199","type":"journal-article","created":{"date-parts":[[2017,3,29]],"date-time":"2017-03-29T02:36:44Z","timestamp":1490755004000},"source":"Crossref","is-referenced-by-count":29,"title":["An Engineered Cell\u2010Instructive Stroma for the Fabrication of a Novel Full Thickness Human Cervix Equivalent In Vitro"],"prefix":"10.1002","volume":"6","author":[{"given":"Vincenza","family":"De Gregorio","sequence":"first","affiliation":[{"name":"Center for Advanced Biomaterials for HealthCare@CRIB Istituto Italiano di Tecnologia  Largo Barsanti e Matteucci 53 80125 Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgia","family":"Imparato","sequence":"additional","affiliation":[{"name":"Center for Advanced Biomaterials for HealthCare@CRIB Istituto Italiano di Tecnologia  Largo Barsanti e Matteucci 53 80125 Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Urciuolo","sequence":"additional","affiliation":[{"name":"Center for Advanced Biomaterials for HealthCare@CRIB Istituto Italiano di Tecnologia  Largo Barsanti e Matteucci 53 80125 Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria L.","family":"Tornesello","sequence":"additional","affiliation":[{"name":"Molecular Biology and Viral Oncology Unit Istituto Nazionale, Tumori IRCCS \u201cFondazione Pascale\u201d  80131 Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clorinda","family":"Annunziata","sequence":"additional","affiliation":[{"name":"Molecular Biology and Viral Oncology Unit Istituto Nazionale, Tumori IRCCS \u201cFondazione Pascale\u201d  80131 Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franco M.","family":"Buonaguro","sequence":"additional","affiliation":[{"name":"Molecular Biology and Viral Oncology Unit Istituto Nazionale, Tumori IRCCS \u201cFondazione Pascale\u201d  80131 Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo A.","family":"Netti","sequence":"additional","affiliation":[{"name":"Center for Advanced Biomaterials for HealthCare@CRIB Istituto Italiano di Tecnologia  Largo Barsanti e Matteucci 53 80125 Naples Italy"},{"name":"Molecular Biology and Viral Oncology Unit Istituto Nazionale, Tumori IRCCS \u201cFondazione Pascale\u201d  80131 Naples Italy"},{"name":"Department of Chemical Materials and Industrial Production (DICMAPI) University of Naples Federico II  P.leTecchio 80 80125 Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,3,29]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2004.02.011"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1735109"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(00)00489-8"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI42297"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02166-10"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1206709"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-03-3214"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2000.00869.x"},{"key":"e_1_2_7_9_1","first-page":"171","volume":"119","author":"McLaughlin\u2010Drubin M. 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