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The effect of the protein is strictly modulated by its interaction with the components of the extracellular matrix. Its therapeutic potential depends on a sustained but controlled release at the microenvironmental level in order to avoid the formation of abnormal blood vessels. In this study, it is hypothesized that the composition of the scaffold plays a key role in modulating the binding, hence the therapeutic effect, of the VEGF released by 3D\u2010cell constructs. It is found that collagen sponges, which poorly bind VEGF, prevent the formation of localized hot spots of excessive concentration, therefore, precluding the development of aberrant angiogenesis despite uncontrolled expression by a genetically engineered population of adipose tissue\u2010derived stromal cells. On the contrary, after seeding on VEGF\u2010binding egg\u2010white scaffolds, the same cell population caused aberrantly enlarged vascular structures after 14 d. Collagen\u2010based engineered tissues also induced a safe and efficient angiogenesis in both the patch itself and the underlying myocardium in rat models. These findings open new perspectives on the control and the delivery of proangiogenic stimuli, and are fundamental for the vascularization of engineered tissues\/organs.<\/jats:p>","DOI":"10.1002\/adhm.201700600","type":"journal-article","created":{"date-parts":[[2017,10,10]],"date-time":"2017-10-10T01:12:42Z","timestamp":1507597962000},"source":"Crossref","is-referenced-by-count":16,"title":["Scaffold Composition Determines the Angiogenic Outcome of Cell\u2010Based Vascular Endothelial Growth Factor Expression by Modulating Its Microenvironmental Distribution"],"prefix":"10.1002","volume":"6","author":[{"given":"Emanuele","family":"Gaudiello","sequence":"first","affiliation":[{"name":"Department of Biomedicine University of Basel  Hebelstrasse 20 CH\u20104031 Basel Switzerland"},{"name":"Department of Surgery University Hospital Basel  Spitalstrasse 21 CH\u20104031 Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ludovic","family":"Melly","sequence":"additional","affiliation":[{"name":"Department of Biomedicine University of Basel  Hebelstrasse 20 CH\u20104031 Basel Switzerland"},{"name":"Department of Surgery University Hospital Basel  Spitalstrasse 21 CH\u20104031 Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Cerino","sequence":"additional","affiliation":[{"name":"Department of Biomedicine University of Basel  Hebelstrasse 20 CH\u20104031 Basel Switzerland"},{"name":"Department of Surgery University Hospital Basel  Spitalstrasse 21 CH\u20104031 Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefano","family":"Boccardo","sequence":"additional","affiliation":[{"name":"Department of Biomedicine University of Basel  Hebelstrasse 20 CH\u20104031 Basel Switzerland"},{"name":"Department of Surgery University Hospital Basel  Spitalstrasse 21 CH\u20104031 Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sasan","family":"Jalili\u2010Firoozinezhad","sequence":"additional","affiliation":[{"name":"Department of Biomedicine University of Basel  Hebelstrasse 20 CH\u20104031 Basel Switzerland"},{"name":"Department of Surgery University Hospital Basel  Spitalstrasse 21 CH\u20104031 Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lifen","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Biomedicine University of Basel  Hebelstrasse 20 CH\u20104031 Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Friedrich","family":"Eckstein","sequence":"additional","affiliation":[{"name":"Department of Biomedicine University of Basel  Hebelstrasse 20 CH\u20104031 Basel Switzerland"},{"name":"Department of Surgery University Hospital Basel  Spitalstrasse 21 CH\u20104031 Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan","family":"Martin","sequence":"additional","affiliation":[{"name":"Department of Biomedicine University of Basel  Hebelstrasse 20 CH\u20104031 Basel Switzerland"},{"name":"Department of Surgery University Hospital Basel  Spitalstrasse 21 CH\u20104031 Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beat A.","family":"Kaufmann","sequence":"additional","affiliation":[{"name":"Department of Biomedicine University of Basel  Hebelstrasse 20 CH\u20104031 Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Banfi","sequence":"additional","affiliation":[{"name":"Department of Biomedicine University of Basel  Hebelstrasse 20 CH\u20104031 Basel Switzerland"},{"name":"Department of Surgery University Hospital Basel  Spitalstrasse 21 CH\u20104031 Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Marsano","sequence":"additional","affiliation":[{"name":"Department of Biomedicine University of Basel  Hebelstrasse 20 CH\u20104031 Basel Switzerland"},{"name":"Department of Surgery University Hospital Basel  Spitalstrasse 21 CH\u20104031 Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,10,10]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrcardio.2009.103"},{"key":"e_1_2_8_2_1","first-page":"109","volume":"1","author":"Losordo D. 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