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Cholera toxin subunit B (CTB) is investigated to facilitate multifunctional glioma\u2010targeted drug delivery by targeting the glycosphingolipid GM1 expressed in the blood\u2013brain barrier (BBB), neovasulature, and glioma cells. When modified on the surface of poly(lactic\u2010co\u2010glycolic acid) (PLGA) nanoparticles (CTB\u2010NPs), CTB fully retains its bioactivity after 24 h incubation in the fresh mouse plasma. The formed protein corona (PC) of CTB\u2010NP and plain PLGA nanoparticles (NP) after incubation in plasma is analyzed using liquid chromatography tandem massspectrometry (nano\u2010LC\u2010MS\/MS). CTB modification does not alter the protein components of the formed PC, macrophage phagocytosis, or pharmacokinetic profiles. CTB\u2010NP can efficiently penetrate the in vitro BBB model and target glioma cells and human umbilical vascular endothelial cells. Paclitaxel is loaded in NP (NP\/PTX) and CTB\u2010NP (CTB\u2010NP\/PTX), and their antiglioma effects are assessed in nude mice bearing intracranial glioma. CTB\u2010NP\/PTX can efficiently induce apoptosis of intracranial glioma cells and ablate neovasulature in vivo, resulting in significant prolongation of survival of nude mice bearing intracranial glioma (34 d) in comparison to those treated with NP\/PTX (29 d), Taxol (24 d), and saline (21 d). The present study suggests a potential multifunctional glioma\u2010targeted drug delivery system enabled by cholera toxin subunit B.<\/jats:p>","DOI":"10.1002\/adhm.201700709","type":"journal-article","created":{"date-parts":[[2017,8,25]],"date-time":"2017-08-25T12:26:41Z","timestamp":1503664001000},"source":"Crossref","is-referenced-by-count":30,"title":["Cholera Toxin Subunit B Enabled Multifunctional Glioma\u2010Targeted Drug Delivery"],"prefix":"10.1002","volume":"6","author":[{"given":"Juan","family":"Guan","sequence":"first","affiliation":[{"name":"School of Basic Medical Sciences and State Key Laboratory of Molecular Engineering of Polymers Fudan University  Shanghai 200032 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zui","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences and State Key Laboratory of Molecular Engineering of Polymers Fudan University  Shanghai 200032 P. R. 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