{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:31:20Z","timestamp":1791253880380,"version":"4.1.0"},"reference-count":63,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,12,28]],"date-time":"2017-12-28T00:00:00Z","timestamp":1514419200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NIH\u2010NCATS UH2NS080691"],"award-info":[{"award-number":["NIH\u2010NCATS UH2NS080691"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The hallmarks of diabetics are insufficient secretion of insulin and dysregulation of glucagon. It is critical to understand release mechanisms of insulin, glucagon, and other hormones from the islets of Langerhans. In spite of remarkable advancements in diabetes research and practice, robust and reproducible models that can measure pancreatic \u03b2\u2010cell function are lacking. Here, a microphysiological analysis platform (MAP) that allows the uniform 3D spheroid formation of pancreatic \u03b2\u2010cell islets, large\u2010scale morphological phenotyping, and gene expression mapping of chronic glycemia and lipidemia development is reported. The MAP enables the scaffold\u2010free formation of densely packed \u03b2\u2010cell spheroids (i.e., multiple array of 110 bioreactors) surrounded with a perfusion flow network inspired by physiologically relevant microenvironment. The MAP permits dynamic perturbations on the \u03b2\u2010cell spheroids and the precise controls of glycemia and lipidemia, which allow us to confirm that cellular apoptosis in the \u03b2\u2010cell spheroid under hyperglycemia and hyperlipidemia is mostly dependent to a reactive oxygen species\u2010induced caspase\u2010mediated pathway. The \u03b2\u2010cells' MAP might provide a potential new map in the pathophysiological mechanisms of \u03b2 cells.<\/jats:p>","DOI":"10.1002\/adhm.201701111","type":"journal-article","created":{"date-parts":[[2017,12,28]],"date-time":"2017-12-28T05:32:45Z","timestamp":1514439165000},"source":"Crossref","is-referenced-by-count":75,"title":["Microphysiological Analysis Platform of Pancreatic Islet \u03b2\u2010Cell Spheroids"],"prefix":"10.1002","volume":"7","author":[{"given":"Sang Hun","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Bioengineering University of California, Berkeley  Berkeley CA 94720 USA"},{"name":"Berkeley Sensor and Actuator Center University of California, Berkeley  Berkeley CA 94720 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SoonGweon","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Bioengineering University of California, Berkeley  Berkeley CA 94720 USA"},{"name":"Berkeley Sensor and Actuator Center University of California, Berkeley  Berkeley CA 94720 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jihwan","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering Hanbat National University  Daejeon 34158 South Korea"},{"name":"Berkeley Sensor and Actuator Center University of California, Berkeley  Berkeley CA 94720 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Byungrae","family":"Cho","sequence":"additional","affiliation":[{"name":"Berkeley Sensor and Actuator Center University of California, Berkeley  Berkeley CA 94720 USA"},{"name":"UC Berkeley and UCSF Joint Graduate Program in Bioengineering  Berkeley\/San Francisco CA 94720 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther J.","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of California, Berkeley  Berkeley CA 94720 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sravani","family":"Kondapavulur","sequence":"additional","affiliation":[{"name":"Department of Bioengineering University of California, Berkeley  Berkeley CA 94720 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongchoul","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering Sogang University  Seoul 04107 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luke P.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Bioengineering University of California, Berkeley  Berkeley CA 94720 USA"},{"name":"Berkeley Sensor and Actuator Center University of California, Berkeley  Berkeley CA 94720 USA"},{"name":"Department of Electrical Engineering and Computer Science University of California, Berkeley  Berkeley CA 94720 USA"},{"name":"Biophysics Graduate Program University of California, Berkeley  Berkeley CA 94720 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,12,28]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00715.2009"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI36209"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006676-199301000-00005"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0603-661"},{"key":"e_1_2_8_5_1","doi-asserted-by":"crossref","first-page":"413","DOI":"10.2337\/diab.37.4.413","volume":"37","author":"Ricordi C.","year":"1987","journal-title":"Diabetes"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.5C6684.2005"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd4231"},{"key":"e_1_2_8_8_1","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1038\/nrg841","volume":"3","author":"Edlund H.","year":"2002","journal-title":"Nat. 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