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However, the application of vaccination still presents two major challenges. One is efficiency of antigen delivery, and the other is dealing with immune tolerance accompanied with tumor development. Lipid zinc phosphate hybrid nanoparticles (LZnP NPs) with a unique material structure can realize efficient delivery of antigens to dendritic cells (DCs) and also serve as an adjuvant to promote immune responses. Herein, ZnP NPs are introduced to load toll\u2010like receptor 4 agonist (monophosphoryl lipid A) and B16F10 melanoma cell\u2010derived tumor lysate (TLS) for vaccination. To regulate immune tolerance, the immune checkpoint antagonist, <jats:sc>d<\/jats:sc>\u2010peptide antagonist (<jats:sup>D<\/jats:sup>PPA\u20101), is involved in treatment. TLS\u2010loaded LZnP nanovaccine can efficiently prime DCs and induce cytotoxic T lymphocytes response. The explored combination treatment further exhibits the anticipated tumor inhibition on therapeutic and prophylactic melanoma models with extended survival time. It demonstrates the possibility to combine TLS\u2010loaded LZnP nanovaccine with <jats:sup>D<\/jats:sup>PPA\u20101 against melanoma and provides support to optimize the combination treatment based on nanovaccine and immune checkpoint therapy.<\/jats:p>","DOI":"10.1002\/adhm.201800837","type":"journal-article","created":{"date-parts":[[2018,12,3]],"date-time":"2018-12-03T03:00:30Z","timestamp":1543806030000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Tumor Lysate\u2010Loaded Lipid Hybrid Nanovaccine Collaborated with an Immune Checkpoint Antagonist for Combination Immunotherapy"],"prefix":"10.1002","volume":"8","author":[{"given":"Xiaomeng","family":"Hu","sequence":"first","affiliation":[{"name":"Tongji School of Pharmacy Huazhong University of Science and Technology  Wuhan 430030 China"},{"name":"Immunology and Allergy Unit Department of Medicine Solna Karolinska Institute and Karolinska University Hospital  Stockholm 171 76 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Wu","sequence":"additional","affiliation":[{"name":"Tongji School of Pharmacy Huazhong University of Science and Technology  Wuhan 430030 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianya","family":"Qin","sequence":"additional","affiliation":[{"name":"Tongji School of Pharmacy Huazhong University of Science and Technology  Wuhan 430030 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Qi","sequence":"additional","affiliation":[{"name":"Tongji School of Pharmacy Huazhong University of Science and Technology  Wuhan 430030 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Qiao","sequence":"additional","affiliation":[{"name":"Tongji School of Pharmacy Huazhong University of Science and Technology  Wuhan 430030 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