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So far, researches focusing on leukocyte behaviors are performed based on both in vivo and in vitro models. The in vivo animal models are usually less controllable due to their extreme complexity and nonignorable species issue. Therefore, many researchers turn to in vitro models. With the advances in micro\/nanofabrication, the microfluidic chip has emerged as a novel platform for model construction in multiple biomedical research fields. Specifically, the microfluidic chip is used to study leukocyte behaviors, due to its incomparable advantages in high throughput, precise control, and flexible integration. Moreover, the small size of the microstructures on the microfluidic chip can better mimic the native microenvironment of leukocytes, which contributes to a more reliable recapitulation. Herein are reviewed the recent advances in microfluidic chip\u2013based leukocyte behavior analysis to provide an overview of this field. Detailed discussions are specifically focused on host defense against pathogens, immunodiagnosis, and immunotherapy studies on microfluidic chips. Finally, the current technical challenges are discussed, as well as possible innovations in this field to improve the related applications.<\/jats:p>","DOI":"10.1002\/adhm.201801406","type":"journal-article","created":{"date-parts":[[2019,1,24]],"date-time":"2019-01-24T06:36:51Z","timestamp":1548311811000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Analysis of Leukocyte Behaviors on Microfluidic Chips"],"prefix":"10.1002","volume":"8","author":[{"given":"Yan","family":"Liu","sequence":"first","affiliation":[{"name":"The Key Laboratory of Biomedical Information Engineering of Ministry of Education School of Life Science and Technology Xi'an Jiaotong University  Xi'an Shaanxi 710049 China"},{"name":"Bioinspired Engineering and Biomechanics Center (BEBC) Xi'an Jiaotong University  Xi'an Shaanxi 710049 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingzhen","family":"Yang","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Biomedical Information Engineering of Ministry of Education School of Life Science and Technology Xi'an Jiaotong University  Xi'an Shaanxi 710049 China"},{"name":"Bioinspired Engineering and Biomechanics Center (BEBC) Xi'an Jiaotong University  Xi'an Shaanxi 710049 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Cao","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Biomedical Information Engineering of Ministry of Education School of Life Science and Technology Xi'an Jiaotong University  Xi'an Shaanxi 710049 China"},{"name":"Bioinspired Engineering and Biomechanics Center (BEBC) Xi'an Jiaotong University  Xi'an Shaanxi 710049 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4351-0222","authenticated-orcid":false,"given":"Feng","family":"Xu","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Biomedical Information Engineering of Ministry of Education School of Life Science and Technology Xi'an Jiaotong University  Xi'an Shaanxi 710049 China"},{"name":"Bioinspired Engineering and Biomechanics Center (BEBC) Xi'an Jiaotong University  Xi'an Shaanxi 710049 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,1,23]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2018.03.011"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06887"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2016.02.002"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.01.001"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/joim.12168"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa4967"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.10140"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1159894"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.4.2155"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.25.816"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2015.03.047"},{"key":"e_1_2_8_12_1","first-page":"1749","volume-title":"13th Int. 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