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While several tissue engineering strategies have been developed to promote cartilage regeneration, it is still challenging to generate functional AC capable of sustaining high load\u2010bearing environments. Here, a new class of cartilage extracellular matrix (cECM)\u2010functionalized alginate bioink is developed for the bioprinting of cartilaginous tissues. The bioinks are 3D\u2010printable, support mesenchymal stem cell (MSC) viability postprinting and robust chondrogenesis in vitro, with the highest levels of COLLII and ACAN expression observed in bioinks containing the highest concentration of cECM. Enhanced chondrogenesis in cECM\u2010functionalized bioinks is also associated with progression along an endochondral\u2010like pathway, as evident by increases in RUNX2 expression and calcium deposition in vitro. The bioinks loaded with MSCs and TGF\u2010\u03b23 are also found capable of supporting robust chondrogenesis, opening the possibility of using such bioinks for direct \u201cprint\u2010and\u2010implant\u201d cartilage repair strategies. Finally, it is demonstrated that networks of 3D\u2010printed polycaprolactone fibers with compressive modulus comparable to native AC can be used to mechanically reinforce these bioinks, with no loss in cell viability. It is envisioned that combinations of such biomaterials can be used in multiple\u2010tool biofabrication strategies for the bioprinting of biomimetic cartilaginous implants.<\/jats:p>","DOI":"10.1002\/adhm.201801501","type":"journal-article","created":{"date-parts":[[2019,1,9]],"date-time":"2019-01-09T07:44:45Z","timestamp":1547019885000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":133,"title":["Fiber Reinforced Cartilage ECM Functionalized Bioinks for Functional Cartilage Tissue Engineering"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5967-7241","authenticated-orcid":false,"given":"Swetha","family":"Rathan","sequence":"first","affiliation":[{"name":"Trinity Centre for Bioengineering Trinity Biomedical Sciences Institute Trinity College Dublin  Dublin 2 Ireland"},{"name":"Department of Mechanical and Manufacturing Engineering School of Engineering Trinity College Dublin  Dublin 2 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