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Pigs act as \u201cmixing vessel\u201d hosts to support and generate new pandemic viruses. Preventing the spread of IAV in pigs effectively can delay or even block cross\u2010species transmission. Universal vaccines based on the highly conserved ectodomain of influenza matrix protein 2 (M2e) have been widely reported, but have not been applied due to inadequate protection. Porcine circovirus type 2 (PCV2) causes immunosuppression and promotes swine influenza virus (SIV) infection. Here, M2e is inserted into capsid protein of PCV2 without burying the neutralizing epitopes and self\u2010assembles to form a bivalent nanovaccine. Inoculation with the nanovaccine induces robust M2e\u2010 and PCV2\u2010specific immune responses. The nanovaccine confers protection against lethal challenges of IAV from different species in mice, and significantly reduces SIV titers in pigs' respiratory tract and blocks SIV transmission. These results indicate that the nanovaccine is an economical and promising PCV2 and universal IAV bivalent vaccine, and it will synergistically and powerfully offer potential ability to block IAV cross\u2010species reassortment and transmission.<\/jats:p>","DOI":"10.1002\/adhm.201900456","type":"journal-article","created":{"date-parts":[[2019,7,3]],"date-time":"2019-07-03T03:26:47Z","timestamp":1562124407000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A Universal Influenza Nanovaccine for \u201cMixing Vessel\u201d Hosts Confers Potential Ability to Block Cross\u2010Species Transmission"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6332-8597","authenticated-orcid":false,"given":"Peiyang","family":"Ding","sequence":"first","affiliation":[{"name":"Henan Provincial Key Laboratory of Animal Immunology Henan Academy of Agricultural Sciences  Zhengzhou 450002 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