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Of all the methods available for sterilization, high\u2010pressure steam sterilization such as autoclaving is the most widely used. While autoclave sterilization minimizes pathogen contamination, it can dramatically impact both structural and biological properties of biomaterials. It has recently been reported that injectable cryogels with shape memory properties hold great promises as 3D macroporous biomimetic scaffolds for biomedical applications including tissue engineering. In this study, the impact of autoclave sterilization on properties of a series of cryogels is measured. Unlike conventional hydrogels, cryogels made of natural polymers demonstrate a strong resilience to autoclave sterilization. This process does not alter either their macrostructural or unique physical properties including syringe injectability. The scaffolds' bioactive sites are preserved and autoclaved cryogels retain their excellent cytological compatibility post\u2010autoclaving. Furthermore, autoclaved cryogels do not trigger a notable activation of primary murine bone marrow\u2010derived dendritic cells suggesting a minimal risk for biomaterial\u2010induced inflammation, which is further confirmed by an in vivo histologic analysis. In summary, these results further demonstrate the huge potential of cryogels in the biomedical field and their capacity to be translated into clinical applications.<\/jats:p>","DOI":"10.1002\/adhm.201900679","type":"journal-article","created":{"date-parts":[[2019,7,26]],"date-time":"2019-07-26T10:29:19Z","timestamp":1564136959000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Autoclavable and Injectable Cryogels for Biomedical Applications"],"prefix":"10.1002","volume":"8","author":[{"given":"Pierre","family":"Villard","sequence":"first","affiliation":[{"name":"Center of Nanotechnology King Abdulaziz University  Jeddah 21589 Saudi Arabia"},{"name":"Department of Chemical Engineering Northeastern University  Boston MA 02215 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahboobeh","family":"Rezaeeyazdi","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Northeastern University  Boston MA 02215 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thibault","family":"Colombani","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Northeastern University  Boston MA 02215 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kasturi","family":"Joshi\u2010Navare","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Northeastern University  Boston MA 02215 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devyesh","family":"Rana","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Northeastern University  Boston MA 02215 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adnan","family":"Memic","sequence":"additional","affiliation":[{"name":"Center of Nanotechnology King Abdulaziz University  Jeddah 21589 Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7704-5608","authenticated-orcid":false,"given":"Sidi A.","family":"Bencherif","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Northeastern University  Boston MA 02215 USA"},{"name":"Department of Bioengineering Northeastern University  Boston MA 02215 USA"},{"name":"John A. 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