{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T03:09:10Z","timestamp":1784171350400,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,3,5]],"date-time":"2020-03-05T00:00:00Z","timestamp":1583366400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2015R1A2A1A10055994"],"award-info":[{"award-number":["2015R1A2A1A10055994"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2016M3A9B6918639"],"award-info":[{"award-number":["2016M3A9B6918639"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008783","name":"National Research Council of Science and Technology","doi-asserted-by":"publisher","award":["CAP\u201016\u201002\u2010KIST"],"award-info":[{"award-number":["CAP\u201016\u201002\u2010KIST"]}],"id":[{"id":"10.13039\/501100008783","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Rapid and simple detection of RNA targets is in high demand due to the growing threat of pandemic viruses. One\u2010step real\u2010time, reverse transcription\u2013polymerase chain reaction (One\u2010step RT\u2010qPCR) using a controlled release system of thermo\u2010responsive materials is developed in this paper to enable high\u2010fidelity RNA analysis as suppressing by\u2010products. The nanocapsules, consisting of upper critical solution temperature (UCST) material and PCR primers, carry or release the primers depending upon the temperature. The UCST nanocapsules are introduced into hydrogel microparticles incorporated with RT primers and then the target RNA is selectively amplified in the microparticle through one\u2010step RT\u2010qPCR. Severe side products are sharply subdued by separating the PCR primers from the RT process by means of the microparticles with nanocapsules. Because the one\u2010step assay is now implemented in a single microparticle, multiple target RNAs can be analyzed in a simple RT\u2010qPCR of multiple particles. Reliable 18\u2010plex one\u2010step RT\u2010qPCR is successfully conducted within 30 min using single\u2010color fluorescent optics. This work also explains the facile fabrication processes used for the thermo\u2010responsive nanocapsules and hydrogel microparticles by the blending polymerization method. Extensible multiplex analysis of influenza virus demonstrates the versatile uses of this one\u2010step RT\u2010qPCR platform.<\/jats:p>","DOI":"10.1002\/adhm.201900790","type":"journal-article","created":{"date-parts":[[2020,3,5]],"date-time":"2020-03-05T10:54:48Z","timestamp":1583405688000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Thermo\u2010Responsive Polymer Capsules in Real\u2010Time One\u2010Step RT\u2010PCR for Highly Multiplex RNA Analysis"],"prefix":"10.1002","volume":"9","author":[{"given":"Junsun","family":"Kim","sequence":"first","affiliation":[{"name":"Center for Molecular Recognition Research Materials and Life Science Research Division Korea Institute of Science and Technology  Seoul 02792 Republic of Korea"},{"name":"Chemical and Biological Engineering Korea University  Seoul 02841 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3210-9388","authenticated-orcid":false,"given":"Seungwon","family":"Jung","sequence":"additional","affiliation":[{"name":"Center for Molecular Recognition Research Materials and Life Science Research Division Korea Institute of Science and Technology  Seoul 02792 Republic of Korea"},{"name":"Applied Chemistry Kyung Hee University  Yongin 17104 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi Yeon","family":"Kim","sequence":"additional","affiliation":[{"name":"Center for Molecular Recognition Research Materials and Life Science Research Division Korea Institute of Science and Technology  Seoul 02792 Republic of Korea"},{"name":"Chemical and Biological Engineering Korea University  Seoul 02841 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bong Kyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Center for Molecular Recognition Research Materials and Life Science Research Division Korea Institute of Science and Technology  Seoul 02792 Republic of Korea"},{"name":"Biomedical Engineering University of Science and Technology (UST)  Daejeon 34113 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soon Hwan","family":"Kwon","sequence":"additional","affiliation":[{"name":"Armed Forces Medical Research Institute  Daejeon 34059 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang Kyung","family":"Kim","sequence":"additional","affiliation":[{"name":"Center for Molecular Recognition Research Materials and Life Science Research Division Korea Institute of Science and Technology  Seoul 02792 Republic of Korea"},{"name":"Biomedical Engineering University of Science and Technology (UST)  Daejeon 34113 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,3,5]]},"reference":[{"key":"e_1_2_5_1_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym9060238"},{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.9.4171"},{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.17.8505-8517.2002"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3532"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc031032"},{"key":"e_1_2_5_3_3","doi-asserted-by":"publisher","DOI":"10.20506\/rst.19.2.1230"},{"key":"e_1_2_5_3_4","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMp1405314"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.28.3.495-503.1990"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.01.019"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1865-1682.2010.01141.x"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/elsc.201000046"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1637\/7417.1"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2004.11.015"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2016.12.019"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2017.06.013"},{"key":"e_1_2_5_7_3","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gni178"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2016.12.013"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/tmi.12857"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2017.03.007"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2004.01.003"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2017.02.049"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.macromol.8b00519"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/admt.201700033"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemmater.6b03548"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0190451"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep22975"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2016.08.057"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2018.01.228"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/bc900491s"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.20.e87"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3lc50655j"},{"key":"e_1_2_5_20_1","first-page":"45","volume":"46","author":"Shamsuri A. A.","year":"2012","journal-title":"Cell. Chem. Technol"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm000121r"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.12.3.367-370.2005"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1039\/C6SC03720H"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fadhm.201900790","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.201900790","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adhm.201900790","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.201900790","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T14:21:11Z","timestamp":1759760471000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.201900790"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,5]]},"references-count":35,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2020,4]]}},"alternative-id":["10.1002\/adhm.201900790"],"URL":"https:\/\/doi.org\/10.1002\/adhm.201900790","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,5]]},"assertion":[{"value":"2019-06-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1900790"}}