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Here, an aptamer\u2010siRNA chimera (Chim)\/polyethyleneimine (PEI)\/5\u2010fluorouracil (5\u2010FU)\/carbon nanotube (CNT)\/collagen membrane is constructed, which could be divided into 15 layers with a thickness of 70\u2013100\u00a0\u00b5m. Sustained release experiments show that the collagen membranes can control 5\u2010FU release for more than 2 weeks. Aptamer\u2010siRNA chimera can specifically bind to gastric cancer cells, enabling targeted delivery of 5\u2010FU and silencing drug\u2010resistant gene. In vitro experiments demonstrated that Chim\/PEI\/5\u2010FU\/CNT nanoparticles promoted the apoptosis of 5\u2010FU\u2010resistant gastric cancer cells, inhibited their invasion and proliferation. Animal experiments show that Chim\/PEI\/5\u2010FU\/CNT\/collagen membrane significantly inhibits the expression of mitogen\u2010activated protein kinase (MAPK), and effectively treats peritoneal dissemination of 5\u2010FU\u2010resistant gastric cancer. Compared with siRNA\/PEI\/5\u2010FU\/CNT group, ki\u201067 proliferation index, and matrix metallopeptidase 9 (MMP9) expression are significantly decreased in the Chim\/PEI\/5\u2010FU\/CNT group, while the proportion of apoptotic cells is markly increased. In conclusion, a chimera\/PEI\/5\u2010FU\/CNT\/collagen membrane is constructed, which can effectively treat peritoneal dissemination of drug\u2010resistant gastric cancer. The study provides a new therapeutic approach for relevant clinical treatment.<\/jats:p>","DOI":"10.1002\/adhm.202001153","type":"journal-article","created":{"date-parts":[[2020,9,16]],"date-time":"2020-09-16T16:28:51Z","timestamp":1600273731000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Construction of Aptamer\u2010siRNA Chimera\/PEI\/5\u2010FU\/Carbon Nanotube\/Collagen Membranes for the Treatment of Peritoneal Dissemination of Drug\u2010Resistant Gastric Cancer"],"prefix":"10.1002","volume":"9","author":[{"given":"Wen","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Pathology The 8th Medical Center Chinese PLA General Hospital  Beijing 100091 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sainan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Hematology The Third Affiliated Hospital of Chongqing Medical University (Gener Hospital)  Chongqing 401120 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Hematology The Third Affiliated Hospital of Chongqing Medical University (Gener Hospital)  Chongqing 401120 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zailin","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Hematology The Third Affiliated Hospital of Chongqing Medical University (Gener Hospital)  Chongqing 401120 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongxu","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pathology The 8th Medical Center Chinese PLA General Hospital  Beijing 100091 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Pu","sequence":"additional","affiliation":[{"name":"Department of Hematology The Third Affiliated Hospital of Chongqing Medical University (Gener Hospital)  Chongqing 401120 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silian","family":"He","sequence":"additional","affiliation":[{"name":"Department of Hematology The Third Affiliated Hospital of Chongqing Medical University (Gener Hospital)  Chongqing 401120 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6446-6692","authenticated-orcid":false,"given":"Yong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Hematology The Third Affiliated Hospital of Chongqing Medical University (Gener Hospital)  Chongqing 401120 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,16]]},"reference":[{"key":"e_1_2_5_1_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000013984"},{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2019.04.034"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2019.01159"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10120-018-0871-5"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2019.10.012"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201902301"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbon.2011.11.043"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2017.04.035"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0885328218814988"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9CC07253E"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/jth.12201"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1560"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.5b01203"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.16407"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2004.04.009"},{"key":"e_1_2_5_12_1","first-page":"7386734","volume":"2019","author":"Kang Y. 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