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Autologous progenitors do not meet the clinical need for regeneration due to their insufficient expansion and differentiation, for which human induced pluripotent stem cells (hiPSCs) are promising alternatives. Airway epithelial patches are engineered by differentiating hiPSC\u2010derived airway progenitors into physiological proportions of ciliated (73.9 \u00b1 5.5%) and goblet (2.1 \u00b1 1.4%) cells on a silk fibroin\u2013collagen vitrigel membrane (SF\u2010CVM) composite biomaterial for transplantation in porcine tracheal defects ex vivo and in vivo. Evaluation of ex vivo tracheal repair using hiPSC\u2010derived SF\u2010CVM patches demonstrate native\u2010like tracheal epithelial metabolism and maintenance of mucociliary epithelium to day 3. In vivo studies demonstrate SF\u2010CVM integration and maintenance of airway patency, showing 80.8 \u00b1 3.6% graft coverage with an hiPSC\u2010derived pseudostratified epithelium and 70.7 \u00b1 2.3% coverage with viable cells, 3 days postoperatively. The utility of bioengineered, hiPSC\u2010derived epithelial patches for airway repair is demonstrated in a short\u2010term preclinical survival model, providing a significant leap for airway reconstruction approaches.<\/jats:p>","DOI":"10.1002\/adhm.202100957","type":"journal-article","created":{"date-parts":[[2021,9,27]],"date-time":"2021-09-27T12:47:11Z","timestamp":1632746831000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Short\u2010Term Preclinical Application of Functional Human Induced Pluripotent Stem Cell\u2010Derived Airway Epithelial Patches"],"prefix":"10.1002","volume":"10","author":[{"given":"Ratna","family":"Varma","sequence":"first","affiliation":[{"name":"Latner Thoracic Surgery Laboratories Toronto General Hospital Research Institute University Health Network Toronto General Hospital University of Toronto  101 College St Toronto ON M5G 0A3 Canada"},{"name":"Institute of Biomedical Engineering 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