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Herein, the development and in vitro validation of a \u201csandwich\u2010type\u201d biofabricated diaphyseal cross\u2010sectional unit, with an outer mechanically robust bioprinted cortical bone shell, encompassing an engineered bone marrow, are reported. Channelized silk fibroin blend sponges derived from <jats:italic>Bombyx mori<\/jats:italic> and <jats:italic>Antheraea assama<\/jats:italic> help in developing compartmentalized endosteum, exhibiting specialized osteoblasts (endosteal niche) and discontinuous endothelium (vascular niche). The cellular cross\u2010talk between these two niches triggered via integrin\u2010mediated cell adhesion, enables in preserving quiescence state of CD34<jats:sup>+<\/jats:sup>\/CD38<jats:sup>\u2212<\/jats:sup> hematopoietic stem cells and their recycling in the engineered marrow. The outer cortical bone strut is developed through multimaterial microextrusion bioprinting strategy. Osteogenically primed mesenchymal stem cells\u2010laden silk fibroin\u2013nano\u2010hydroxyapatite bioink is bioprinted alongside paramagnetic Fe\u2010doped bioactive glass\u2013polycaprolactone blend thermoplastic ink, reinforcing it for mechanical stability. Pulsed magnetic field actuation positively influences the osteogenic commitment and maturation of the bioprinted constructs via mechanotransductory route. Therefore, the assembled engineered marrow and bioprinted cortical shell hold promise as potential orthobiologic substitutes toward atrophic nonunion repairs.<\/jats:p>","DOI":"10.1002\/adhm.202102031","type":"journal-article","created":{"date-parts":[[2021,12,8]],"date-time":"2021-12-08T21:20:07Z","timestamp":1638998407000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Silk\u2010Based Bioengineered Diaphyseal Cortical Bone Unit Enclosing an Implantable Bone Marrow toward Atrophic Nonunion Grafting"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9794-8196","authenticated-orcid":false,"given":"Joseph Christakiran","family":"Moses","sequence":"first","affiliation":[{"name":"Biomaterials and Tissue Engineering Laboratory Department of Biosciences and Bioengineering Indian Institute of Technology Guwahati  Guwahati Assam 781039 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Souradeep","family":"Dey","sequence":"additional","affiliation":[{"name":"Centre for Nanotechnology Indian Institute of Technology Guwahati  Guwahati Assam 781039 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0066-8337","authenticated-orcid":false,"given":"Ashutosh","family":"Bandyopadhyay","sequence":"additional","affiliation":[{"name":"Biomaterials and Tissue Engineering Laboratory Department of Biosciences and Bioengineering Indian Institute of Technology Guwahati  Guwahati Assam 781039 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manoj","family":"Agarwala","sequence":"additional","affiliation":[{"name":"GNRC Institute of Medical Sciences (formerly known as Guwahati Neurological Research Centre)  Guwahati Assam 781039 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3936-4621","authenticated-orcid":false,"given":"Biman B.","family":"Mandal","sequence":"additional","affiliation":[{"name":"Biomaterials and Tissue Engineering Laboratory Department of Biosciences and Bioengineering Indian Institute of Technology Guwahati  Guwahati Assam 781039 India"},{"name":"Centre for Nanotechnology Indian Institute of Technology Guwahati  Guwahati Assam 781039 India"},{"name":"School of Health Science and Technology Indian Institute of Technology Guwahati  Guwahati Assam 781039 India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,12,17]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jor.2016.10.003"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/3715964"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874325001105010193"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-1383(08)70003-2"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1779"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201400758"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1039\/C5TB02644J"},{"key":"e_1_2_8_6_3","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2018.03.028"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1805440115"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.07.042"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1705258"},{"key":"e_1_2_8_9_1","first-page":"72","volume":"9","author":"Mousavi S. 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