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Pancreatic duct\u2010like organoids (PDLOs) derived from human pluripotent stem cells (PSCs) or pancreatic cancer patient\u2010derived organoids (PDOs) provide unique tools to study early and late stage dysplasia and to foster personalized medicine. However, such advanced systems are neither rapidly nor easily accessible and require an in vivo niche to study tumor formation and interaction with the stroma. Here, the establishment of the porcine urinary bladder (PUB) is revealed as an advanced organ culture model for shaping an ex vivo pancreatic niche. This model allows pancreatic progenitor cells to enter the ductal and endocrine lineages, while PDLOs further mature into duct\u2010like tissue. Accordingly, the PUB offers an ex vivo platform for earliest pancreatic dysplasia and cancer if PDLOs feature <jats:italic>KRAS<jats:sup>G12D<\/jats:sup><\/jats:italic> mutations. Finally, it is demonstrated that PDOs\u2010on\u2010PUB i) resemble primary pancreatic cancer, ii) preserve cancer subtypes, iii) enable the study of niche epithelial crosstalk by spiking in pancreatic stellate and immune cells into the grafts, and finally iv) allow drug testing. In summary, the PUB advances the existing pancreatic cancer models by adding feasibility, complexity, and customization at low cost and high flexibility.<\/jats:p>","DOI":"10.1002\/adhm.202102345","type":"journal-article","created":{"date-parts":[[2022,2,3]],"date-time":"2022-02-03T17:58:32Z","timestamp":1643911112000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Organoids at the PUB: The Porcine Urinary Bladder Serves as a Pancreatic Niche for Advanced Cancer Modeling"],"prefix":"10.1002","volume":"11","author":[{"given":"Michael Karl","family":"Melzer","sequence":"first","affiliation":[{"name":"Department of Urology Ulm University  Ulm 89081 Germany"},{"name":"Department of Internal Medicine I Ulm University  Ulm 89081 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Breunig","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I Ulm 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