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In this work, a highly versatile ultrasound\u2010assisted biofabrication (UAB) approach is demonstrated that utilizes radiation forces generated by superimposing ultrasonic bulk acoustic waves to rapidly organize arrays of cells and other biomaterial additives within single and multilayered hydrogel constructs. UAB is used in conjunction with a novel hybrid bioink system, comprising of cartilage\u2010forming cells (human adipose\u2010derived stem cells or chondrocytes) and additives to promote cell adhesion (collagen microaggregates or polycaprolactone microfibers) encapsulated within gelatin methacryloyl (GelMA) hydrogels, to fabricate cartilaginous tissue constructs featuring bulk anisotropy. The hybrid matrices fabricated under the appropriate synergistic thermo\u2010reversible and photocrosslinking conditions demonstrate enhanced mechanical stiffness, stretchability, strength, construct shape fidelity and aligned encapsulated cell morphology and collagen II secretion in long\u2010term culture. Hybridization of UAB is also shown with extrusion and stereolithography printing to fabricate constructs featuring 3D perfusable channels for vasculature combined with a crisscross or circumferential organization of cells and adhesive bioadditives, which is relevant for further translation of UAB toward complex physiological\u2010scale biomimetic tissue fabrication.<\/jats:p>","DOI":"10.1002\/adhm.202102351","type":"journal-article","created":{"date-parts":[[2022,1,14]],"date-time":"2022-01-14T14:23:52Z","timestamp":1642170232000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Multiscale Anisotropic Tissue Biofabrication via Bulk Acoustic Patterning of Cells and Functional Additives in Hybrid Bioinks"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6107-6848","authenticated-orcid":false,"given":"Parth","family":"Chansoria","sequence":"first","affiliation":[{"name":"Edward P. Fitts Department of Industrial and Systems Engineering and Comparative Medicine Institute North Carolina State University  Raleigh NC 27695 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suleman","family":"Asif","sequence":"additional","affiliation":[{"name":"Edward P. Fitts Department of Industrial and Systems Engineering and Comparative Medicine Institute North Carolina State University  Raleigh NC 27695 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nithin","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Molecular Biomedical Sciences and Comparative Medicine Institute North Carolina State University  Raleigh NC 27695 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9751-3192","authenticated-orcid":false,"given":"Jorge","family":"Piedrahita","sequence":"additional","affiliation":[{"name":"Department of Molecular Biomedical Sciences and Comparative Medicine Institute North Carolina State University  Raleigh NC 27695 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0215-2847","authenticated-orcid":false,"given":"Rohan A.","family":"Shirwaiker","sequence":"additional","affiliation":[{"name":"Edward P. Fitts Department of Industrial and Systems Engineering Comparative Medicine Institute Joint Department of Biomedical Engineering and Department of Mechanical and Aerospace Engineering North Carolina State University  Raleigh NC 27695 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,1,27]]},"reference":[{"key":"e_1_2_11_1_1","doi-asserted-by":"publisher","DOI":"10.1080\/10255842.2012.739167"},{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2001.280.5.H2222"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.201200483"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.medengphy.2019.09.021"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2012.0330"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2008.0652"},{"key":"e_1_2_11_7_1","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1016\/B978-0-323-07307-3.10036-9","volume-title":"Pediatr. 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