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Herein, a strategy is proposed to obtain local genital tract immunity against\u00a0<jats:italic>C. trachomatis\u00a0<\/jats:italic>through parenteral prime and sublingual boost. Subcutaneous administration of chlamydia CTH522 subunit vaccine loaded in the adjuvant CAF01 is combined with sublingual administration of CTH522 loaded in a novel thermosensitive and mucoadhesive hydrogel. Briefly, a ternary optimized hydrogel (OGEL) with desirable biological and physicochemical properties is obtained using artificial intelligence techniques. This formulation exhibits a high gel strength and a strong mucoadhesive, adhesive and cohesive nature. The thermosensitive properties of the hydrogel facilitate application under the tongue. Meanwhile the fast gelation at body temperature together with rapid antigen release should avoid CTH522 leakage by swallowing and increase the contact with sublingual tissue, thus promoting absorption.\u00a0In vivo\u00a0studies demonstrate that parenteral\u2010sublingual prime\u2010boost immunization, using CAF01 and OGEL as CTH522 vaccine carriers, shows a tendency to increase cellular (Th1\/Th17) immune responses when compared to mucosal or parenteral vaccination alone. Furthermore, parenteral prime with\u00a0CAF01\/CTH522\u00a0followed by sublingual boosting with OGEL\/CTH522 elicits a local IgA response in the genital tract.<\/jats:p>","DOI":"10.1002\/adhm.202102508","type":"journal-article","created":{"date-parts":[[2022,2,6]],"date-time":"2022-02-06T03:14:10Z","timestamp":1644117250000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Sublingual Boosting with a Novel Mucoadhesive Thermogelling Hydrogel Following Parenteral CAF01 Priming as a Strategy Against <i>Chlamydia trachomatis<\/i>"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1784-6355","authenticated-orcid":false,"given":"Lorena","family":"Garcia\u2010del Rio","sequence":"first","affiliation":[{"name":"Departamento de Farmacolog\u00eda Farmacia y Tecnolog\u00eda Farmac\u00e9utica Grupo I+D Farma (GI\u20101645) Agrupaci\u00f3n Estrat\u00e9gica de Materiales (AeMat) Facultad de Farmacia Universidade de Santiago de Compostela  Santiago de Compostela 15782 Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria de Santiago de Compostela (IDIS) IDIS Research Institute  Santiago de Compostela 15706 Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7576-2013","authenticated-orcid":false,"given":"Patricia","family":"Diaz\u2010Rodriguez","sequence":"additional","affiliation":[{"name":"Departamento de Farmacolog\u00eda Farmacia y Tecnolog\u00eda Farmac\u00e9utica Grupo I+D Farma (GI\u20101645) Agrupaci\u00f3n Estrat\u00e9gica de Materiales (AeMat) Facultad de Farmacia Universidade de Santiago de Compostela  Santiago de Compostela 15782 Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria de Santiago de Compostela (IDIS) IDIS Research Institute  Santiago de Compostela 15706 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