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Natural scaffolds of extracellular matrix or fibrin contain fibrils with nanoscale dimensions, but how platelets specifically respond to the topography and architecture of fibrous materials is still incompletely understood. Here, planar and nanofiber scaffolds are fabricated from native fibrinogen to characterize the morphology of adherent platelets and activation markers for phosphatidylserine exposure and <jats:italic>\u03b1<\/jats:italic>\u2010granule secretion by confocal fluorescence microscopy and scanning electron microscopy. Different fibrinogen topographies equally support the spreading and \u03b1\u2010granule secretion of washed platelets. In contrast, preincubation of the scaffolds with plasma diminishes platelet spreading on planar fibrinogen surfaces but not on nanofibers. The data show that the enhanced interactions of platelets with nanofibers result from a higher locally accessible surface area, effectively increasing the ligand density for integrin\u2010mediated responses. Overall, fibrinogen nanofibers direct platelets toward robust adhesion formation and <jats:italic>\u03b1<\/jats:italic>\u2010granule secretion while minimizing their procoagulant activity. Similar results on fibrinogen\u2010coated polydimethylsiloxane substrates with micrometer\u2010sized 3D features suggest that surface topography could be used more generally to steer blood\u2010materials interactions on different length scales for enhancing the initial wound healing steps.<\/jats:p>","DOI":"10.1002\/adhm.202200249","type":"journal-article","created":{"date-parts":[[2022,5,8]],"date-time":"2022-05-08T03:39:11Z","timestamp":1651981151000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Nanofiber Topographies Enhance Platelet\u2010Fibrinogen Scaffold Interactions"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3055-8075","authenticated-orcid":false,"given":"Martin","family":"Kenny","sequence":"first","affiliation":[{"name":"School of Pharmacy and Biomolecular Sciences Royal College of Surgeons in Ireland (RCSI)  123 St Stephen's Green Dublin D02 YN77 Ireland"},{"name":"Irish Centre for Vascular Biology Royal College of Surgeons in Ireland (RCSI)  123 St Stephen's Green Dublin D02 YN77 Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8400-7909","authenticated-orcid":false,"given":"Stephani","family":"Stamboroski","sequence":"additional","affiliation":[{"name":"Institute for Biophysics University of Bremen  Otto\u2010Hahn\u2010Allee 1 Bremen 28359 Germany"},{"name":"Fraunhofer Institute for Manufacturing Technology and Advanced Materials (IFAM)  Wiener Strasse 12 Bremen 28359 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reem","family":"Taher","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Biomolecular Sciences Royal College of Surgeons in Ireland (RCSI)  123 St Stephen's Green Dublin D02 YN77 Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7140-3275","authenticated-orcid":false,"given":"Dorothea","family":"Br\u00fcggemann","sequence":"additional","affiliation":[{"name":"Institute for Biophysics University of Bremen  Otto\u2010Hahn\u2010Allee 1 Bremen 28359 Germany"},{"name":"MAPEX Center for Materials and Processes University of Bremen  Bremen 28359 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5699-1160","authenticated-orcid":false,"given":"Ingmar","family":"Schoen","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Biomolecular Sciences Royal College of Surgeons in Ireland (RCSI)  123 St Stephen's Green Dublin D02 YN77 Ireland"},{"name":"Irish Centre for Vascular Biology Royal College of Surgeons in Ireland (RCSI)  123 St Stephen's Green Dublin D02 YN77 Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,5,18]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2014.12.068"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.2217\/nnm.15.82"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.6b00291"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jddst.2018.01.009"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2018.00099"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2019.06.022"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2019.06.020"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41569-018-0110-0"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2005.01365.x"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1159\/000339613"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00067.2017"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-03-773614"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)49910-9"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-10-054015"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1322917111"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-bioeng-062117-121215"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2017.182972"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-00885-x"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-01-200345"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2014.10.003"},{"key":"e_1_2_9_21_1","first-page":"3532","volume":"13","author":"Nurden A. 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