{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T02:31:43Z","timestamp":1787797903080,"version":"build-2784847793"},"reference-count":68,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2022,7,29]],"date-time":"2022-07-29T00:00:00Z","timestamp":1659052800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010198","name":"Ministerio de Econom\u00eda, Industria y Competitividad, Gobierno de Espa\u00f1a","doi-asserted-by":"publisher","award":["RTC\u20102016\u20105451\u20101"],"award-info":[{"award-number":["RTC\u20102016\u20105451\u20101"]}],"id":[{"id":"10.13039\/501100010198","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008061","name":"Fundaci\u00f3n Mutua Madrile\u00f1a","doi-asserted-by":"publisher","award":["FMM\u2010AP17196\u20102019"],"award-info":[{"award-number":["FMM\u2010AP17196\u20102019"]}],"id":[{"id":"10.13039\/100008061","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["DTS19\/00145"],"award-info":[{"award-number":["DTS19\/00145"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["DTS21\/00098"],"award-info":[{"award-number":["DTS21\/00098"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011011","name":"Junta de Andaluc\u00eda","doi-asserted-by":"publisher","award":["B\u2010TEP\u2010026\u2010UGR18"],"award-info":[{"award-number":["B\u2010TEP\u2010026\u2010UGR18"]}],"id":[{"id":"10.13039\/501100011011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011011","name":"Junta de Andaluc\u00eda","doi-asserted-by":"publisher","award":["IE2017\u20105537"],"award-info":[{"award-number":["IE2017\u20105537"]}],"id":[{"id":"10.13039\/501100011011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011011","name":"Junta de Andaluc\u00eda","doi-asserted-by":"publisher","award":["P18\u2010RT\u20101653"],"award-info":[{"award-number":["P18\u2010RT\u20101653"]}],"id":[{"id":"10.13039\/501100011011","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Osteoarthritis is a disease with a great socioeconomic impact and mainly affects articular cartilage, a tissue with reduced self\u2010healing capacity. In this work, 3D printed 1,4 butanediol thermoplastic polyurethane (b\u2010TPUe) scaffolds are functionalized and infrapatellar mesenchymal stem cells are used as the cellular source. Since b\u2010TPUe is a biomaterial with mechanical properties similar to cartilage, but it does not provide the desired environment for cell adhesion, scaffolds are functionalized with two methods, one based on collagen type I and the other in 1\u2010pyrenebutiric acid (PBA) as principal components. Alamar Blue and confocal assays display that PBA functionalized scaffolds support higher cell adhesion and proliferation for the first 21 days. However, collagen type I functionalization induces higher proliferation rates and similar cell viability than the PBA method. Further, both functionalization methods induce extracellular matrix synthesis, and the presence of chondrogenic markers (Sox9, Col2a, and Acan). Finally, SEM images probe that functionalized 3D printed scaffolds present much better cell\/biomaterial interactions than controls and confirm early chondrogenesis. These results indicate that the two methods of functionalization in the highly hydrophobic b\u2010TPUe enhance the cell\u2010biomaterial interactions and the improvement in the chondro\u2010inductive properties, which have great potential for application in cartilage tissue engineering.<\/jats:p>","DOI":"10.1002\/adhm.202200251","type":"journal-article","created":{"date-parts":[[2022,7,20]],"date-time":"2022-07-20T11:49:40Z","timestamp":1658317780000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Chondro\u2010Inductive b\u2010TPUe\u2010Based Functionalized Scaffolds for Application in Cartilage Tissue Engineering"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2298-658X","authenticated-orcid":false,"given":"Daniel","family":"Mart\u00ednez\u2010Moreno","sequence":"first","affiliation":[{"name":"Instituto de Investigaci\u00f3n Biosanitaria de Granada (ibs.GRANADA) University Hospitals of Granada\u2010University of Granada  Granada E\u201018071 Spain"},{"name":"Biopathology and Regenerative Medicine Institute (IBIMER) Centre for Biomedical Research University of Granada  Granada E\u201018100 Spain"},{"name":"Department of Human Anatomy and Embryology Faculty of Medicine University of Granada  Granada E\u201018016 Spain"},{"name":"Excellence Research Unit \u201cModeling Nature\u201d (MNat) University of Granada  Granada E\u201018016 Spain"},{"name":"BioFab i3D\u2010 Biofabrication and 3D (bio)printing laboratory University of Granada  Granada E\u201018100 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Desir\u00e9","family":"Venegas\u2010Bustos","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Biosanitaria de Granada (ibs.GRANADA) University Hospitals of Granada\u2010University of Granada  Granada E\u201018071 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermo","family":"Rus","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Biosanitaria de Granada (ibs.GRANADA) University Hospitals of Granada\u2010University of Granada  Granada E\u201018071 Spain"},{"name":"Excellence Research Unit \u201cModeling Nature\u201d (MNat) University of Granada  Granada E\u201018016 Spain"},{"name":"Department of Structural Mechanics University of Granada Polit\u00e9cnico de Fuentenueva  Granada E\u201018071 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"G\u00e1lvez\u2010Mart\u00edn","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Pharmaceutical Technology Faculty of Pharmacy University of Granada  Granada E\u201018071 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gema","family":"Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Biosanitaria de Granada (ibs.GRANADA) University Hospitals of Granada\u2010University of Granada  Granada E\u201018071 Spain"},{"name":"Biopathology and Regenerative Medicine Institute (IBIMER) Centre for Biomedical Research University of Granada  Granada E\u201018100 Spain"},{"name":"Department of Human Anatomy and Embryology Faculty of Medicine University of Granada  Granada E\u201018016 Spain"},{"name":"Excellence Research Unit \u201cModeling Nature\u201d (MNat) University of Granada  Granada E\u201018016 Spain"},{"name":"BioFab i3D\u2010 Biofabrication and 3D (bio)printing laboratory University of Granada  Granada E\u201018100 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4996-8261","authenticated-orcid":false,"given":"Juan Antonio","family":"Marchal","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Biosanitaria de Granada (ibs.GRANADA) University Hospitals of Granada\u2010University of Granada  Granada E\u201018071 Spain"},{"name":"Biopathology and Regenerative Medicine Institute (IBIMER) Centre for Biomedical Research University of Granada  Granada E\u201018100 Spain"},{"name":"Department of Human Anatomy and Embryology Faculty of Medicine University of Granada  Granada E\u201018016 Spain"},{"name":"Excellence Research Unit \u201cModeling Nature\u201d (MNat) University of Granada  Granada E\u201018016 Spain"},{"name":"BioFab i3D\u2010 Biofabrication and 3D (bio)printing laboratory University of Granada  Granada E\u201018100 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,7,29]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.2217\/17460751.3.1.1"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.1995.1.151"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11926-013-0375-6"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2010.198"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-017-2613-0"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rchot.2015.10.005"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2014.157"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000360156"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/1947603514546740"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2011.0155"},{"key":"e_1_2_10_11_1","first-page":"431","volume":"105","author":"Rad M. R.","year":"2015","journal-title":"J. Biomed. Mater. Res., Part B"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-006-8234-x"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2019.109883"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms12118217"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1163\/156856208X396074"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.langmuir.6b04237"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-008-3393-6"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym10121304"},{"key":"e_1_2_10_19_1","first-page":"641","volume":"6","author":"Keshel S. H.","year":"2011","journal-title":"Int. J. Nanomed."},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.polymdegradstab.2015.10.022"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/7293193"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/14653240600855905"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1159\/000469703"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201400018"},{"key":"e_1_2_10_25_1","volume":"8","author":"Wang C.","year":"2019","journal-title":"Adv. Healthcare Mater."},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-010-4173-7"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2015.12.087"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm100199m"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2021.111933"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2016.01.019"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0964-8305(02)00051-3"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1177\/0194599811419092"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2019.02.044"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.6b07872"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2017.03.303"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2010.246"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201500032"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1116\/1.4943046"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-102546-8.00005-4"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/btm2.10192"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.11.070"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.095786"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.02.033"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.3109\/02652048.2014.950712"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(99)00026-5"},{"key":"e_1_2_10_46_1","unstructured":"I.Sallent H.Ctor Capella\u2010Monson\u00eds D. I.Zeugolis https:\/\/doi.org\/10.1007\/978\u20101\u20104939\u20109095\u20105_3(accessed: August 2021)."},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6TA07211A"},{"key":"e_1_2_10_48_1","first-page":"56","volume-title":"Genetic and Metabolic Disease in Pediatrics: Butterworths International","author":"Byers P. H.","year":"2014"},{"key":"e_1_2_10_49_1","first-page":"511","volume":"70","author":"Dietze S.","year":"2018","journal-title":"Clin. Hemorheol. Microcirc."},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202001847"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.08.019"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21207744"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.26981"},{"key":"e_1_2_10_54_1","volume":"29","author":"Zhang Y.","year":"2019","journal-title":"Adv. Funct. Mater."},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matpr.2018.04.181"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2021.10.002"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2019.03.011"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/0049-0172(91)90036-Y"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmst.2020.03.022"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-011-0911-3"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.21330"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2012.04.017"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199909)180:3<314::AID-JCP2>3.0.CO;2-Y"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.12659\/AJCR.920688"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2012.10.007"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2018.02.024"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plgene.2015.03.001"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v035a15"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202200251","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adhm.202200251","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202200251","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T16:33:28Z","timestamp":1759768408000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.202200251"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,29]]},"references-count":68,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2022,10]]}},"alternative-id":["10.1002\/adhm.202200251"],"URL":"https:\/\/doi.org\/10.1002\/adhm.202200251","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,29]]},"assertion":[{"value":"2022-01-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2200251"}}