{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T00:58:46Z","timestamp":1792198726482,"version":"4.4.0"},"reference-count":68,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2022,9,23]],"date-time":"2022-09-23T00:00:00Z","timestamp":1663891200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,9,23]],"date-time":"2022-09-23T00:00:00Z","timestamp":1663891200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000185","name":"Defense Advanced Research Projects Agency","doi-asserted-by":"publisher","award":["D20AC00002"],"award-info":[{"award-number":["D20AC00002"]}],"id":[{"id":"10.13039\/100000185","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DGE1745016"],"award-info":[{"award-number":["DGE1745016"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DGE2140739"],"award-info":[{"award-number":["DGE2140739"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000038","name":"U.S. Food and Drug Administration","doi-asserted-by":"publisher","award":["R01FD006582"],"award-info":[{"award-number":["R01FD006582"]}],"id":[{"id":"10.13039\/100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000009","name":"Foundation for the National Institutes of Health","doi-asserted-by":"publisher","award":["1F30HL154728"],"award-info":[{"award-number":["1F30HL154728"]}],"id":[{"id":"10.13039\/100000009","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000009","name":"Foundation for the National Institutes of Health","doi-asserted-by":"publisher","award":["K99HL155777"],"award-info":[{"award-number":["K99HL155777"]}],"id":[{"id":"10.13039\/100000009","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Soft tissue injuries such as volumetric muscle loss (VML) are often too large to heal normally on their own, resulting in scar formation and functional deficits. Decellularized extracellular matrix (dECM) scaffolds placed into these wounds have shown the ability to modulate the immune response and drive constructive healing. This provides a potential solution for functional tissue regeneration, however, these acellular dECM scaffolds are challenging to fabricate into complex geometries. 3D bioprinting is uniquely positioned to address this, being able to create patient\u2010specific scaffolds based on clinical 3D imaging data. Here, a process to use freeform reversible embedding of suspended hydrogels (FRESH) 3D bioprinting and computed tomography (CT) imaging to build large volume, patient\u2010specific dECM patches (\u224812\u00a0\u00d7\u00a08\u00a0\u00d7\u00a02\u00a0cm) for implantation into canine VML wound models is developed. Quantitative analysis shows that these dECM patches are dimensionally accurate and conformally adapt to the surface of complex wounds. Finally, this approach is extended\u00a0to a human VML injury to demonstrate the fabrication of clinically relevant dECM scaffolds with precise control over fiber alignment and micro\u2010architecture. Together these advancements represent a step towards an improved, clinically translatable, patient\u2010specific treatment for soft tissue defects from trauma, tumor resection, and other surgical procedures.<\/jats:p>","DOI":"10.1002\/adhm.202200866","type":"journal-article","created":{"date-parts":[[2022,9,5]],"date-time":"2022-09-05T09:04:37Z","timestamp":1662368677000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["3D Bioprinted Patient\u2010Specific Extracellular Matrix Scaffolds for Soft Tissue Defects"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4788-6086","authenticated-orcid":false,"given":"Anne","family":"Behre","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering Carnegie Mellon University  Pittsburgh PA 15213 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8193-0039","authenticated-orcid":false,"given":"Joshua W.","family":"Tashman","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Carnegie Mellon University  Pittsburgh PA 15213 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1659-1269","authenticated-orcid":false,"given":"Caner","family":"Dikyol","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Carnegie Mellon University  Pittsburgh PA 15213 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6978-303X","authenticated-orcid":false,"given":"Daniel J.","family":"Shiwarski","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Carnegie Mellon University  Pittsburgh PA 15213 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8597-1874","authenticated-orcid":false,"given":"Raphael J.","family":"Crum","sequence":"additional","affiliation":[{"name":"McGowan Institute for Regenerative Medicine University of Pittsburgh  Pittsburgh PA 15219 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0164-6802","authenticated-orcid":false,"given":"Scott A.","family":"Johnson","sequence":"additional","affiliation":[{"name":"McGowan Institute for Regenerative Medicine University of Pittsburgh  Pittsburgh PA 15219 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Remya","family":"Kommeri","sequence":"additional","affiliation":[{"name":"McGowan Institute for Regenerative Medicine University of Pittsburgh  Pittsburgh PA 15219 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George S.","family":"Hussey","sequence":"additional","affiliation":[{"name":"McGowan Institute for Regenerative Medicine University of Pittsburgh  Pittsburgh PA 15219 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3555-0689","authenticated-orcid":false,"given":"Stephen F.","family":"Badylak","sequence":"additional","affiliation":[{"name":"McGowan Institute for Regenerative Medicine University of Pittsburgh  Pittsburgh PA 15219 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3338-5456","authenticated-orcid":false,"given":"Adam W.","family":"Feinberg","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Carnegie Mellon University  Pittsburgh PA 15213 USA"},{"name":"McGowan Institute for Regenerative Medicine University of Pittsburgh  Pittsburgh PA 15219 USA"},{"name":"Department of Materials Science &amp; Engineering Carnegie Mellon University  Pittsburgh PA 15213 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,23]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-396983-5.00015-6"},{"key":"e_1_2_10_2_1","unstructured":"Volumetric Muscle Loss\u202f: JAAOS \u2013 Journal of the American Academy of Orthopaedic Surgeons https:\/\/www.journals.lww.com\/jaaos\/Fulltext\/2011\/02001\/Volumetric_Muscle_Loss.7.aspx(accessed: February 2022)."},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002281-200003000-00010"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.berh.2006.12.004"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/TA.0b013e318163b875"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOT.0b013e31802f78fb"},{"key":"e_1_2_10_7_1","unstructured":"2010 Report of the 2009 Statistics of Plastic Surgery Statistics: American Society of Plastic Surgeons 2010 https:\/\/www.plasticsurgery.org\/documents\/News\/Statistics\/2009\/plastic\u2010surgery\u2010statistics\u2010full\u2010report\u20102009.pdf"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-020-06476-5"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.prs.0000260595.85557.41"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering7030085"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3008085"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.06.039"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2016.06.047"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/npjregenmed.2016.8"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2010.0169"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2012.0475"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41578-018-0023-x"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201801168"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2016.11.068"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.12.014"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2958"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0178540"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202000734"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4935"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2016.01.013"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2018.03.040"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201600483"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-29968-5"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2019.03.036"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.1500758"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aav9051"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2017.05.002"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2016.07.020"},{"key":"e_1_2_10_34_1","first-page":"2105883","author":"Samandari M.","year":"2021","journal-title":"Adv. Mater."},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2011.11.1029"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mri.2012.05.001"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ohx.2020.e00170"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202103469"},{"key":"e_1_2_10_39_1","first-page":"19","author":"Grogan J. R. H. B. F.","year":"2011","journal-title":"J Am Acad Orthop Surg"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/anbr.202100068"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2008.09.013"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2006.12.519"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/ac58be"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.0c01133"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-011-1185-7"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10439-016-1592-8"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2013.04.036"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/wnan.1254"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/admt.201600003"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3210"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2008.11.014"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201600240"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2008.03.031"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2018.0280"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2019.0126"},{"key":"e_1_2_10_56_1","unstructured":"eCFR\u202f:: 21 CFR 3.2 \u2013 Definitions https:\/\/www.ecfr.gov\/current\/title\u201021\/chapter\u2010I\/subchapter\u2010A\/part\u20103\/subpart\u2010A\/section\u20103.2(accessed: July 2022)."},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)68438-9"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.37349"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19051319"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2016.0538"},{"key":"e_1_2_10_61_1","first-page":"57","volume":"8","author":"Chuen Wong K.","year":"2016","journal-title":"Orthop Res Rev"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2017.11.008"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.2217\/3dp-2016-0001"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ohx.2018.02.001"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.06.060"},{"key":"e_1_2_10_66_1","unstructured":"\u201cMedical CT | 3D CAD Model Library | GrabCAD\u201d https:\/\/www.grabcad.com\/library\/medical\u2010ct\u20101(accessed: November 2021)."},{"key":"e_1_2_10_67_1","unstructured":"\u201cSCALED FIGURE | 3D CAD Model Library | GrabCAD\u201d https:\/\/www.grabcad.com\/library\/scaled\u2010figure"},{"key":"e_1_2_10_68_1","unstructured":"\u201cMakerGear M3ID | 3D CAD Model Library | GrabCAD\u201d https:\/\/www.grabcad.com\/library\/makergear\u2010m3id\u20101(accessed: November 2021)."}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202200866","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adhm.202200866","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202200866","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202200866","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T15:11:11Z","timestamp":1759763471000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.202200866"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,23]]},"references-count":68,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["10.1002\/adhm.202200866"],"URL":"https:\/\/doi.org\/10.1002\/adhm.202200866","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,23]]},"assertion":[{"value":"2022-04-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2200866"}}