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However, long\u2010term caries prophylaxis cannot be maintained because of the negative effects derived from the technical sensitivity and disadvantages of sealing materials. Herein, a new strategy is proposed to occlude fossae by amyloid\u2010mediated biomimetic remineralization. In contrast to conventional inward blocking from the outside of fossae, amyloid\u2010mediated biomimetic mineralization delivers an amyloid\u2010like protein nanofilm into the deepest zone of the fossae and induces the formation of remineralized enamel inside. Such assembly from lysozyme conjugated with poly (ethylene glycol) enriches the interface with strongly bonded ionsand directs in situ nucleation to achieve enamel epitaxial growth. Not only is the structure of the enamel\u2010like crystalline hydroxyapatite layer but also its mechanical stability is similar to that of natural enamel. Furthermore, the layer shows good biocompatibility and antibacterial properties. On the basis of the findings, it is demonstrated that amyloid\u2010like protein aggregation may provide an enamel remineralization strategy to modify the current clinically available methods of pit and fissure sealing and shows great promise in preventing caries.<\/jats:p>","DOI":"10.1002\/adhm.202200872","type":"journal-article","created":{"date-parts":[[2022,7,23]],"date-time":"2022-07-23T00:15:02Z","timestamp":1658535302000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Amyloid\u2010Mediated Remineralization in Pit and Fissure for Caries Preventive Therapy"],"prefix":"10.1002","volume":"11","author":[{"given":"Xiaoxue","family":"Yang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Military Stomatology &amp; National Clinical Research Center for Oral Diseases &amp; Shaanxi Clinical Research Center for Oral Diseases Department of Preventive Dentistry School of Stomatology Fourth Military Medical University  Xi'an Shaanxi 710032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Guo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Military Stomatology &amp; 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