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Herein it is reported for the first time that hyperbranched polylysine (HPL) shows antifungal activities against\n                    <jats:italic>Candida<\/jats:italic>\n                    , especially for drug\u2010sensitive and MDR\n                    <jats:italic>C. albicans<\/jats:italic>\n                    strains, and broad\u2010spectrum antibacterial activities against both Gram\u2010negative and Gram\u2010positive bacteria. The high antimicrobial activities are ascribed to the high charge density and compact size of the globular structure of HPL. The in vitro antifungal activities of HPL3 are further enhanced by the modification of amine groups to form guanylated polylysines (HPL3\u2010Gxs). Similar to antimicrobial peptides (AMPs), HPLs and HPL3\u2010Gxs interact with and lyse the membranes of microbes, which mitigates the emergence of drug resistance. HPLs and HPL3\u2010Gxs demonstrate excellent in vivo antimicrobial efficacies against both lethal\n                    <jats:italic>C. albicans<\/jats:italic>\n                    challenge in the invasive candidiasis model and lethal Methicillin resistant\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    challenge in the peritonitis model, and have potentials as broad\u2010spectrum antimicrobials.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202201091","type":"journal-article","created":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T09:06:15Z","timestamp":1656666375000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Guanylated Hyperbranched Polylysines with High In Vitro and In Vivo Antifungal Activity"],"prefix":"10.1002","volume":"11","author":[{"given":"Xiao","family":"Liu","sequence":"first","affiliation":[{"name":"Key Laboratory of Polymer Ecomaterials Changchun Institute of Applied Chemistry Chinese Academy of Sciences  5625 Renmin Street Changchun Jilin 130022 P. 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