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PDXs remain difficult to implement in vitro, particularly in configurations that enable both detailed cellular analysis and high\u2010throughput screening (HTS). Complex multilineage co\u2010cultures with stromal fibroblasts, endothelium, and other cellular and structural components of the tumor microenvironment (TME) further complicate ex vivo implementation. Herein,\u00a0the culture of multiple prostate cancer (PCa)\u2010derived PDX models as 3D clusters within engineered biomimetic hydrogel matrices, in a HTS\u2010compatible multiwell microfluidic format, alongside bone marrow\u2010derived stromal cells and a perfused endothelial channel. Polymeric hydrogel matrices are customized for each cell type, enabling cell survival in vitro and facile imaging across all conditions. PCa PDXs demonstrate unique morphologies and reliance on TME partners, retention of known phenotype, and expected sensitivity or resistance to standard PCa therapeutics. This novel integration of technologies provides a fully human model, and expands the information to be gathered from each specimen, while avoiding the time and labor involved with animal\u2010based testing.<\/jats:p>","DOI":"10.1002\/adhm.202201434","type":"journal-article","created":{"date-parts":[[2022,12,3]],"date-time":"2022-12-03T01:19:32Z","timestamp":1670030372000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["A 3D Perfusable Platform for In Vitro Culture of Patient Derived Xenografts"],"prefix":"10.1002","volume":"12","author":[{"given":"Lindsey K.","family":"Sablatura","sequence":"first","affiliation":[{"name":"Department of BioSciences Rice University  Houston TX 77005 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin M.","family":"Bircsak","sequence":"additional","affiliation":[{"name":"MIMETAS US Inc  Gaithersburg MD 20878 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Shepherd","sequence":"additional","affiliation":[{"name":"Department of Genitourinary Medical Oncology Research Division of Cancer Medicine The University of Texas MD Anderson Cancer Center  Houston TX 77030 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madhavi","family":"Bathina","sequence":"additional","affiliation":[{"name":"Division of Health Equities Department of Population Sciences City of Hope Comprehensive Cancer Center  Duarte CA 91010 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karla","family":"Queiroz","sequence":"additional","affiliation":[{"name":"MIMETAS B.V.  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